Directed enzyme evolution has revolutionized the rapid development of enzymes with
desired properties. However, the lack of a high-throughput method to identify the most …

This review article concerns the production of recombinant antibody fragments for
applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very …

Small single-chain variable fragments (scFv) are promising biomolecules to inhibit and
neutralize toxins and to act as antivenoms. In this work, we aimed to produce a functional …

This research reported a hydrogel loaded with the ethanolic and methanolic extracts of
Eupatorium glutinosum Lam. The E. glutinosum extracts were characterized by …

Caldimonas manganoxidans is a Gram-negative, thermophilic, bioplastic-producing
bacterium that is a promising strain to overcome the drawbacks of existing bioplastic …

The synthesis of insulin using recombinant Pichia pastoris represents a significant
advancement in biotechnology with profound implications for healthcare. This review …

Recent years have seen the development of high-accuracy and high-throughput genetic
manipulation techniques, which have greatly improved our understanding of genetically …

Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the
flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and …

The introduction of plasmids into Agrobacterium cells is one of the key steps in the
Agrobacterium-mediated transformation of plants for gene editing applications. Depending …

摘要L-丝氨酸是一种具有重要生理学功能的中性氨基酸, 广泛应用在食品, 化妆品和医药等领域.
为构建L-丝氨酸的生产菌株, 对Escherichia coli MG1655 进行了系统的代谢工程改造 …