CRISPR tools to control gene expression in bacteria

A Vigouroux, D Bikard - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
SUMMARY CRISPR-Cas systems have been engineered as powerful tools to control gene
expression in bacteria. The most common strategy relies on the use of Cas effectors …

Gradients in gene essentiality reshape antibacterial research

AM Hogan, ST Cardona - FEMS Microbiology Reviews, 2022 - academic.oup.com
Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels

F Depardieu, D Bikard - Methods, 2020 - Elsevier
The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …

Construction of a gene knockdown system based on catalytically inactive (“dead”) Cas9 (dCas9) in Staphylococcus aureus

C Zhao, X Shu, B Sun - Applied and environmental microbiology, 2017 - Am Soc Microbiol
There has been an absence of an efficient method of gene knockdown in the important
human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The …

Bacterial cellular engineering by genome editing and gene silencing

N Nakashima, K Miyazaki - International journal of molecular sciences, 2014 - mdpi.com
Genome editing is an important technology for bacterial cellular engineering, which is
commonly conducted by homologous recombination-based procedures, including gene …

Genome-wide CRISPRi-based identification of targets for decoupling growth from production

S Li, CB Jendresen, J Landberg… - ACS Synthetic …, 2020 - ACS Publications
Growth decoupling can be used to optimize microbial production of biobased compounds by
inhibiting excess biomass formation and redirect carbon flux from growth to product …

Variability of Bacterial Essential Genes Among Closely Related Bacteria: The Case of Escherichia coli

E Martínez-Carranza, H Barajas, LD Alcaraz… - Frontiers in …, 2018 - frontiersin.org
The definition of bacterial essential genes has been widely pursued using different
approaches. Their study has impacted several fields of research such as synthetic biology …

Downregulation of yidC in Escherichia coli by Antisense RNA Expression Results in Sensitization to Antibacterial Essential Oils Eugenol and Carvacrol

SD Patil, R Sharma, S Srivastava, NK Navani… - PLoS …, 2013 - journals.plos.org
Background The rising drug resistance in pathogenic bacteria and inefficiency of current
antibiotics to meet clinical requirements has augmented the need to establish new and …

Rapid prototyping of microbial cell factories via genome-scale engineering

T Si, H Xiao, H Zhao - Biotechnology advances, 2015 - Elsevier
Advances in reading, writing and editing genetic materials have greatly expanded our ability
to reprogram biological systems at the resolution of a single nucleotide and on the scale of a …

New and improved tools and methods for enhanced biosynthesis of natural products in microorganisms

Z Wang, PC Cirino - Current opinion in biotechnology, 2016 - Elsevier
Highlights•Inducible gene suppression tools provide a powerful alternative to gene
deletions.•Implementing dynamic gene regulation balances fluxes and reduces metabolic …