AM Hogan, ST Cardona - FEMS Microbiology Reviews, 2022 - academic.oup.com
Essential genes encode the processes that are necessary for life. Until recently, commonly applied binary classifications left no space between essential and non-essential genes. In …
The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …
C Zhao, X Shu, B Sun - Applied and environmental microbiology, 2017 - Am Soc Microbiol
There has been an absence of an efficient method of gene knockdown in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The …
N Nakashima, K Miyazaki - International journal of molecular sciences, 2014 - mdpi.com
Genome editing is an important technology for bacterial cellular engineering, which is commonly conducted by homologous recombination-based procedures, including gene …
Growth decoupling can be used to optimize microbial production of biobased compounds by inhibiting excess biomass formation and redirect carbon flux from growth to product …
The definition of bacterial essential genes has been widely pursued using different approaches. Their study has impacted several fields of research such as synthetic biology …
Background The rising drug resistance in pathogenic bacteria and inefficiency of current antibiotics to meet clinical requirements has augmented the need to establish new and …
Advances in reading, writing and editing genetic materials have greatly expanded our ability to reprogram biological systems at the resolution of a single nucleotide and on the scale of a …
Z Wang, PC Cirino - Current opinion in biotechnology, 2016 - Elsevier
Highlights•Inducible gene suppression tools provide a powerful alternative to gene deletions.•Implementing dynamic gene regulation balances fluxes and reduces metabolic …