The design and application of DNA-editing enzymes as base editors
KL Rallapalli, AC Komor - Annual review of biochemistry, 2023 - annualreviews.org
DNA-editing enzymes perform chemical reactions on DNA nucleobases. These reactions
can change the genetic identity of the modified base or modulate gene expression. Interest …
can change the genetic identity of the modified base or modulate gene expression. Interest …
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
Revolutionizing genetic disease treatment: recent technological advances in base editing
Treating human genetic diseases has posed a significant and longstanding challenge to the
medical community with traditional approaches often limited by their targeting scope …
medical community with traditional approaches often limited by their targeting scope …
Split complementation of base editors to minimize off-target edits
X Xiong, K Liu, Z Li, FN Xia, XM Ruan, X He, JF Li - Nature Plants, 2023 - nature.com
Base editors (BEs) empower the efficient installation of beneficial or corrective point
mutations in crop and human genomes. However, conventional BEs can induce …
mutations in crop and human genomes. However, conventional BEs can induce …
Myo-differentiation reporter screen reveals NF-Y as an activator of PAX3–FOXO1 in rhabdomyosarcoma
MW Sroka, D Skopelitis, MW Vermunt… - Proceedings of the …, 2023 - National Acad Sciences
Recurrent chromosomal rearrangements found in rhabdomyosarcoma (RMS) produce the
PAX3–FOXO1 fusion protein, which is an oncogenic driver and a dependency in this …
PAX3–FOXO1 fusion protein, which is an oncogenic driver and a dependency in this …
A chemically controlled Cas9 switch enables temporal modulation of diverse effectors
CRISPR–Cas9 has yielded a plethora of effectors, including targeted transcriptional
activators, base editors and prime editors. Current approaches for inducibly modulating …
activators, base editors and prime editors. Current approaches for inducibly modulating …
Structure-guided design of a potent and specific inhibitor against the genomic mutator APOBEC3A
JC Serrano, D von Trentini, KN Berríos… - ACS chemical …, 2022 - ACS Publications
Nucleic acid structure plays a critical role in governing the selectivity of DNA-and RNA-
modifying enzymes. In the case of the APOBEC3 family of cytidine deaminases, these …
modifying enzymes. In the case of the APOBEC3 family of cytidine deaminases, these …
Expanded palette of RNA base editors for comprehensive RBP-RNA interactome studies
HC Medina-Munoz, E Kofman, P Jagannatha… - Nature …, 2024 - nature.com
RNA binding proteins (RBPs) are key regulators of RNA processing and cellular function.
Technologies to discover RNA targets of RBPs such as TRIBE (targets of RNA binding …
Technologies to discover RNA targets of RBPs such as TRIBE (targets of RNA binding …
TadA orthologs enable both cytosine and adenine editing of base editors
S Zhang, B Yuan, J Cao, L Song, J Chen, J Qiu… - Nature …, 2023 - nature.com
Cytidine and adenosine deaminases are required for cytosine and adenine editing of base
editors respectively, and no single deaminase could enable concurrent and comparable …
editors respectively, and no single deaminase could enable concurrent and comparable …