Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

H Li, Y Xie, F Chen, H Bai, L Xiu, X Zhou… - Chemical Society …, 2023 - pubs.rsc.org
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

The CRISPR–Cas toolbox for analytical and diagnostic assay development

Y Tang, L Gao, W Feng, C Guo, Q Yang, F Li… - Chemical Society …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …

CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants

Z Chen, C Wu, Y Yuan, Z Xie, T Li, H Huang… - Journal of …, 2023 - Springer
Since the end of 2019, a highly contagious disease caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called …

CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

Y Li, S Man, S Ye, G Liu, L Ma - Comprehensive Reviews in …, 2022 - Wiley Online Library
Food safety is one of the biggest public issues occurring around the world. Microbiological,
chemical, and physical hazards can lead to food safety issues, which may occur at all stages …

State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis

B Lu, Z Guo, K Zhong, T Osire, Y Sun, L Jiang - Trends in Food Science & …, 2023 - Elsevier
Background Food safety is a major global public health issue. The majority of traditional
culture-based methods have lengthy detection times, require sophisticated sample …

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

SY Wang, YC Du, DX Wang, JY Ma, AN Tang… - Analytica Chimica …, 2021 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …

Isothermal nucleic acid amplification for food safety analysis

X Xia, H Yang, J Cao, J Zhang, Q He, R Deng - TrAC Trends in Analytical …, 2022 - Elsevier
Rapid assays allowing for on-site or in-field testing can enable us to detect food
contamination in a timely manner, thus facilitating the insurance of food safety. Isothermal …

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

X Zhang, Y Shi, G Chen, D Wu, Y Wu, G Li - Small Methods, 2022 - Wiley Online Library
Infectious pathogens cause severe human illnesses and great deaths per year worldwide.
Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing …

[HTML][HTML] Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle

S Sun, H Liu, Y Hu, Y Wang, M Zhao, Y Yuan, Y Han… - Bioactive Materials, 2023 - Elsevier
Skeletal muscle disorders have posed great threats to health. Selective delivery of drugs
and oligonucleotides to skeletal muscle is challenging. Aptamers can improve targeting …

CRISPR/Cas-engineered technology: Innovative approach for biosensor development

TS Zavvar, Z Khoshbin, M Ramezani… - Biosensors and …, 2022 - Elsevier
On-site and real-time clinical monitoring have been progressed dramatically by integrating
biosensor science with portable digital electronic technology. Clustered regularly …