Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

Following fertilization, the two specified gametes must unite to create an entirely new
organism. The genome is initially transcriptionally quiescent, allowing the zygote to be …

Background Exosomes have emerged as vital biomarkers of multiple cancers and contain
abundant circular RNAs (circRNAs). However, the potential for exosomal circRNAs to be …

Models of gene control have emerged from genetic and biochemical studies, with limited
consideration of the spatial organization and dynamics of key components in living cells. We …

Abstract N 6-methyladenosine (m 6 A) is the most abundant internal modification in
eukaryotic messenger RNAs (mRNAs), and plays important roles in cell differentiation and …

We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of
RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales …

Expansions of short nucleotide repeats produce several neurological and neuromuscular
disorders including Huntington disease, muscular dystrophy, and amyotrophic lateral …

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …

Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging
methods and quantitative analysis, we examine transcriptional bursting in living Drosophila …

Signals in many biological processes can be amplified by recruiting multiple copies of
regulatory proteins to a site of action. Harnessing this principle, we have developed a …