Validation of Reference Genes for Gene Expression Studies in Virus-Infected Nicotiana benthamiana Using Quantitative Real-Time PCR

D Liu, L Shi, C Han, J Yu, D Li, Y Zhang - 2012 - journals.plos.org
Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …

Transcriptome analysis in different rice cultivars provides novel insights into desiccation and salinity stress responses

R Shankar, A Bhattacharjee, M Jain - Scientific reports, 2016 - nature.com
Drought and salinity are the major environmental factors that affect rice productivity.
Comparative transcriptome analysis between tolerant and sensitive rice cultivars can …

Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development

X Han, M Lu, Y Chen, Z Zhan, Q Cui, Y Wang - 2012 - journals.plos.org
Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

Development of reference genes for horticultural plants

U Ahmed, Q Xie, X Shi, B Zheng - Critical Reviews in Plant …, 2022 - Taylor & Francis
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is extensively
applied technique to investigate the transcript abundance of target genes in various …

Identification of Optimal Reference Genes for Expression Analysis in Radish (Raphanus sativus L.) and Its Relatives Based on Expression Stability

M Duan, J Wang, X Zhang, H Yang, H Wang… - Frontiers in plant …, 2017 - frontiersin.org
Radish (Raphanus sativus) is an important cruciferous root crop with a close relationship to
Chinese cabbage (Brassica rapa). RT-qPCR is used extensively to evaluate the expression …

Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

LLB Amorim, JRC Ferreira-Neto, JP Bezerra-Neto… - Plant Methods, 2018 - Springer
Background Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to
drought and salt stresses, efforts have been directed to identifying genes and pathways that …

Systematic identification of reference genes for qRT-PCR of Ardisia kteniophylla A. DC under different experimental conditions and for anthocyanin-related genes …

W Wang, X Zhang, X Xu, X Xu, L Fu… - Frontiers in Plant …, 2023 - frontiersin.org
Ardisia kteniophylla A. DC, widely known as folk medicinal herb and ornamental plant, has
been extensively investigated due to its unique leaf color, anti-cancer and other …

Public-transcriptome-database-assisted selection and validation of reliable reference genes for qRT-PCR in rice

Z Zhao, Z Zhang, Z Ding, H Meng, R Shen… - Science China Life …, 2020 - Springer
Accurate quantitative reverse transcription PCR (qRT-PCR) requires reliable reference
genes whose expression does not vary in different tissues and developmental stages …

Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla

Y He, H Yan, W Hua, Y Huang, Z Wang - Frontiers in plant science, 2016 - frontiersin.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the …

Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in Nicotiana benthamiana upon Single Infections by 11 Positive-Sense …

G Zhang, Z Zhang, Q Wan, H Zhou, M Jiao, H Zheng… - Plants, 2023 - mdpi.com
Quantitative real-time PCR (RT-qPCR) is a widely used method for studying alterations in
gene expression upon infections caused by diverse pathogens such as viruses. Positive …