CRISPR/Cas genome editing and precision plant breeding in agriculture

K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants

SSA Zaidi, A Mahas, H Vanderschuren, MM Mahfouz - Genome biology, 2020 - Springer
To meet increasing global food demand, breeders and scientists aim to improve the yield
and quality of major food crops. Plant diseases threaten food security and are expected to …

Applications and potential of genome editing in crop improvement

Y Zhang, K Massel, ID Godwin, C Gao - Genome biology, 2018 - Springer
Genome-editing tools provide advanced biotechnological techniques that enable the
precise and efficient targeted modification of an organism's genome. Genome-editing …

Gene editing in plants: progress and challenges

Y Mao, JR Botella, Y Liu, JK Zhu - National Science Review, 2019 - academic.oup.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

CS Lin, CT Hsu, LH Yang, LY Lee, JY Fu… - Plant biotechnology …, 2018 - Wiley Online Library
Plant protoplasts are useful for assessing the efficiency of clustered regularly interspaced
short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) mutagenesis. We …

[HTML][HTML] Engineering herbicide resistance via prime editing in rice

H Butt, GS Rao, K Sedeek, R Aman… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
Engineering herbicide resistance via prime editing in rice - PMC Back to Top Skip to main
content NIH NLM Logo Access keys NCBI Homepage MyNCBI Homepage Main Content …

A detailed landscape of CRISPR-Cas-mediated plant disease and pest management

S Karmakar, P Das, D Panda, K Xie, MJ Baig, KA Molla - Plant Science, 2022 - Elsevier
Genome editing technology has rapidly evolved to knock-out genes, create targeted genetic
variation, install precise insertion/deletion and single nucleotide changes, and perform large …

Targeted DNA insertion in plants

OX Dong, PC Ronald - … of the National Academy of Sciences, 2021 - National Acad Sciences
Conventional methods of DNA sequence insertion into plants, using Agrobacterium-
mediated transformation or microprojectile bombardment, result in the integration of the DNA …

Programmable RNA detection with CRISPR-Cas12a

SR Rananaware, EK Vesco, GM Shoemaker… - Nature …, 2023 - nature.com
Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …

Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice

Z Ali, A Shami, K Sedeek, R Kamel, A Alhabsi… - Communications …, 2020 - nature.com
Precise genome editing by systems such as clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency …