To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

The ability to multiplex PCR by probe color and melting temperature (Tm) greatly expands
the power of real-time analysis. Simple hybridization probes with only a single fluorescent …

A microvolume fluorometer integrated with a thermal cycler was used to acquire DNA
melting curves during polymerase chain reaction by fluorescence monitoring of the double …

Rapid cycle DNA amplification was continuously monitored by three different fluorescence
techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green …

This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …

Background: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was
recently introduced as a homogeneous, closed-tube method of genotyping that does not …

Reverse transcription (RT) followed by a polymerase chain reaction (PCR) represents the
most powerful technology to amplify and detect trace amounts of mRNA (Heid et al., 1996; …

Experimental and commercial microvolume fluorimeters with rapid temperature control are
described. Fluorescence optics adopted from flow cytometry were used to interrogate 1–10 …

The polymerase chain reaction (PCR) has become an integral tool for the molecular
biologist. It represents a workhorse in the molecular biology laboratory whilst also proving to …

Methods of monitoring hybridization during polymerase chain reaction are disclosed. These
methods are achieved With rapid thermal cycling and use of double stranded DNA dyes or …