Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology

C Berger, N Premaraj, RBG Ravelli, K Knoops… - Nature …, 2023 - nature.com
Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

A technical introduction to transmission electron microscopy for soft‐matter: Imaging, possibilities, choices, and technical developments

LE Franken, K Grünewald, EJ Boekema, MCA Stuart - Small, 2020 - Wiley Online Library
With a significant role in material sciences, physics,(soft matter) chemistry, and biology, the
transmission electron microscope is one of the most widely applied structural analysis tool to …

Serial Lift-Out: sampling the molecular anatomy of whole organisms

OH Schiøtz, CJO Kaiser, S Klumpe, DR Morado… - Nature …, 2024 - nature.com
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

FR Wagner, R Watanabe, R Schampers, D Singh… - Nature protocols, 2020 - nature.com
Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …

[HTML][HTML] Recent advances and current trends in cryo-electron microscopy

M Guaita, SC Watters, S Loerch - Current opinion in structural biology, 2022 - Elsevier
All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Serialized on-grid lift-in sectioning for tomography (SOLIST) enables a biopsy at the nanoscale

HTD Nguyen, G Perone, N Klena, R Vazzana… - Nature …, 2024 - nature.com
Cryo-focused ion beam milling has substantially advanced our understanding of molecular
processes by opening windows into cells. However, applying this technique to complex …

Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography

E Villa, M Schaffer, JM Plitzko, W Baumeister - Current opinion in structural …, 2013 - Elsevier
Cryo-electron tomography (CET) is ideally suited for bridging the resolution gap between
molecular and cellular structural studies. However, CET is limited to a sample thickness …

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

S Gorelick, G Buckley, G Gervinskas, TK Johnson… - Elife, 2019 - elifesciences.org
Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the
structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo …

Multidimensional visualization of the dynamic evolution of Li metal via in situ/operando methods

S Lang, M Colletta, MR Krumov… - Proceedings of the …, 2023 - National Acad Sciences
The growing demands for high-energy density electrical energy storage devices stimulate
the coupling of conversion-type cathodes and lithium (Li) metal anodes. While promising …

A focused ion beam milling and lift-out approach for site-specific preparation of frozen-hydrated lamellas from multicellular organisms

J Mahamid, R Schampers, H Persoon… - Journal of structural …, 2015 - Elsevier
Cryo-electron tomography provides 3D views of cellular architecture with molecular
resolution. A principal limitation of cryo-transmission electron microscopy performed on cells …