Cells rely on complex molecular networks to perceive and process external and internal
signals into tailored responses. These cellular abilities can be augmented and modified by …

Synthetic biology is an established but ever-growing interdisciplinary field of research
currently revolutionizing biomedicine studies and the biotech industry. The engineering of …

Synthetic messenger RNA (mRNA) has been focused on as an emerging application for
mRNA-based therapies and vaccinations. Recently, synthetic circular RNAs (circRNAs) …

Isolation of specific cell types, including pluripotent stem cell (PSC)-derived populations, is
frequently accomplished using cell surface antigens expressed by the cells of interest …

Synthetic biological circuits are designed to regulate gene expressions to control cell
function. To date, these circuits often use DNA-delivery methods, which may lead to random …

Synthetic messenger RNA (mRNA) tools often use pseudouridine and 5-methyl cytidine as
substitutions for uridine and cytidine to avoid the immune response and cytotoxicity induced …

CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease
expression triggers off-target effects and even in vivo immune responses. Inspired by …

Predictably regulating protein expression levels to improve recombinant protein production
has become an important tool, but is still rarely applied to engineer mammalian cells. We …

The field of mammalian synthetic biology is expanding quickly, and technologies for
engineering large synthetic gene circuits are increasingly accessible. However, for …

The bottom-up assembly of biological components in synthetic biology has contributed to a
better understanding of natural phenomena and the development of new technologies for …