Challenging bioanalyses with capillary electrophoresis

CJ Kristoff, L Bwanali, LM Veltri, GP Gautam… - Analytical …, 2019 - ACS Publications
This report covers advances in capillary electrophoresis (CE) from January 2018 through
September 2019. A summary of the literature during this time period is insightful. A search …

Microfluidic approaches for the analysis of protein–protein interactions in solution

WE Arter, A Levin, G Krainer, TPJ Knowles - Biophysical reviews, 2020 - Springer
Exploration and characterisation of the human proteome is a key objective enabling a
heightened understanding of biological function, malfunction and pharmaceutical design …

Targeting nucleic acid phase transitions as a mechanism of action for antimicrobial peptides

T Sneideris, NA Erkamp, H Ausserwöger… - Nature …, 2023 - nature.com
Antimicrobial peptides (AMPs), which combat bacterial infections by disrupting the bacterial
cell membrane or interacting with intracellular targets, are naturally produced by a number of …

Surface electrostatics govern the emulsion stability of biomolecular condensates

TJ Welsh, G Krainer, JR Espinosa, JA Joseph… - Nano …, 2022 - ACS Publications
Liquid–liquid phase separation underlies the formation of biological condensates.
Physically, such systems are microemulsions that in general have a propensity to fuse and …

α-Synuclein oligomers form by secondary nucleation

CK Xu, G Meisl, EA Andrzejewska, G Krainer… - Nature …, 2024 - nature.com
Oligomeric species arising during the aggregation of α-synuclein are implicated as a major
source of toxicity in Parkinson's disease, and thus a major potential drug target. However …

Quaternization of Vinyl/Alkynyl Pyridine Enables Ultrafast Cysteine‐Selective Protein Modification and Charge Modulation

MJ Matos, CD Navo, T Hakala, X Ferhati… - Angewandte Chemie …, 2019 - Wiley Online Library
Quaternized vinyl‐and alkynyl‐pyridine reagents were shown to react in an ultrafast and
selective manner with several cysteine‐tagged proteins at near‐stoichiometric quantities …

Scalable integration of nano-, and microfluidics with hybrid two-photon lithography

O Vanderpoorten, Q Peter, PK Challa… - Microsystems & …, 2019 - nature.com
Nanofluidic devices have great potential for applications in areas ranging from renewable
energy to human health. A crucial requirement for the successful operation of nanofluidic …

A rapid and controllable acoustothermal microheater using thin film surface acoustic waves

Y Wang, Q Zhang, R Tao, D Chen, J Xie… - Sensors and Actuators A …, 2021 - Elsevier
Temperature control within a microreactor is critical for biochemical and biomedical
applications. Recently acoustothermal heating using surface acoustic wave (SAW) devices …

Rapid structural, kinetic, and immunochemical analysis of alpha-synuclein oligomers in solution

WE Arter, CK Xu, M Castellana-Cruz, TW Herling… - Nano …, 2020 - ACS Publications
Oligomers comprised of misfolded proteins are implicated as neurotoxins in the
pathogenesis of protein misfolding conditions such as Parkinson's and Alzheimer's …

The role of shear forces in primary and secondary nucleation of amyloid fibrils

E Axell, J Hu, M Lindberg, AJ Dear… - Proceedings of the …, 2024 - National Acad Sciences
Shear forces affect self-assembly processes ranging from crystallization to fiber formation.
Here, the effect of mild agitation on amyloid fibril formation was explored for four peptides …