[HTML][HTML] DNA barcoding in herbal medicine: Retrospective and prospective

S Chen, X Yin, J Han, W Sun, H Yao, J Song… - Journal of Pharmaceutical …, 2023 - Elsevier
DNA barcoding has been widely used for herb identification in recent decades, enabling
safety and innovation in the field of herbal medicine. In this article, we summarize recent …

High-resolution DNA melting analysis for simple and efficient molecular diagnostics

GH Reed, JO Kent, CT Wittwer - Pharmacogenomics, 2007 - Taylor & Francis
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …

mlo‐based powdery mildew resistance in hexaploid bread wheat generated by a non‐transgenic TILLING approach

J Acevedo‐Garcia, D Spencer… - Plant biotechnology …, 2017 - Wiley Online Library
Wheat is one of the most widely grown cereal crops in the world and is an important food
grain source for humans. However, wheat yields can be reduced by many abiotic and biotic …

High‐Resolution Melting Analysis (HRMA)—More than just sequence variant screening

RHAM Vossen, E Aten, A Roos… - Human mutation, 2009 - Wiley Online Library
Transition of the double‐stranded DNA molecule to its two single strands, DNA denaturation
or melting, has been used for many years to study DNA structure and composition. Recent …

High‐resolution DNA melting analysis: advancements and limitations

CT Wittwer - Human mutation, 2009 - Wiley Online Library
Recent advances in fluorescent dyes, methods, instruments and software for DNA melting
analysis have created versatile new tools for variant scanning and genotyping. High …

DNA barcoding: A modern age tool for detection of adulteration in food

N Nehal, B Choudhary, A Nagpure… - Critical reviews in …, 2021 - Taylor & Francis
Globalization of the food trade requires precise and exact information about the origin,
methods of production, transformation technologies, authentication, and the traceability of …

The diagnostic protocol for hereditary spherocytosis‐2021 update

Y Wu, L Liao, F Lin - Journal of Clinical Laboratory Analysis, 2021 - Wiley Online Library
Background Hereditary spherocytosis (HS), a commonly encountered hereditary hemolytic
disease, is mostly inherited in an autosomal dominant manner. The clinical manifestations in …

High resolution melting (HRM) for high-throughput genotyping—limitations and caveats in practical case studies

M Słomka, M Sobalska-Kwapis, M Wachulec… - International journal of …, 2017 - mdpi.com
High resolution melting (HRM) is a convenient method for gene scanning as well as
genotyping of individual and multiple single nucleotide polymorphisms (SNPs). This rapid …

Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis

J Montgomery, CT Wittwer, R Palais, L Zhou - Nature protocols, 2007 - nature.com
This protocol permits the simultaneous mutation scanning and genotyping of PCR products
by high-resolution DNA melting analysis. This is achieved using asymmetric PCR performed …

Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes

MG Herrmann, JD Durtschi, LK Bromley… - Clinical …, 2006 - academic.oup.com
Background: DNA melting analysis for genotyping and mutation scanning of PCR products
by use of high-resolution instruments with special “saturation” dyes has recently been …