CRISPR-Cas has revolutionized genetic modification with its comparative simplicity and
accuracy, and it can be used even at the genomic level. Microalgae are excellent feedstocks …

Life-like systems need to maintain a basal metabolism, which includes importing a variety of
building blocks required for macromolecule synthesis, exporting dead-end products, and …

We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

The ultimate microscope, directed at a cell, would reveal the dynamics of all the cell's
components with atomic resolution. In contrast to their real-world counterparts …

Genomically minimal cells, such as JCVI-syn3. 0, offer a platform to clarify genes underlying
core physiological processes. Although this minimal cell includes genes essential for …

All cells are encapsulated by a lipid membrane that facilitates their interactions with the
environment. How cells manage diverse mixtures of lipids, which dictate membrane property …

In synthetic biology, biological cells and processes are dismantled and reassembled to
make novel systems that do useful things. Designs are encoded by deoxyribonucleic acid …

Possessing only essential genes, a minimal cell can reveal mechanisms and processes that
are critical for the persistence and stability of life,. Here we report on how an engineered …

Abstract Model organisms such as the Gram‐positive bacterium Bacillus subtilis have been
studied intensively for decades. However, even for model organisms, no function has been …

The vast majority of genomic sequences are automatically annotated using various software
programs. The accuracy of these annotations depends heavily on the very few manual …