Serial crystallography, born from groundbreaking experiments at the Linac Coherent Light
Source in 2009, has evolved into a pivotal technique in structural biology. Initially pioneered …

This perspective review describes emerging techniques and future opportunities for time-
resolved serial femtosecond crystallography (TR-SFX) experiments using high repetition …

New synthetic hybrid materials and their increasing complexity have placed growing
demands on crystal growth for single-crystal X-ray diffraction analysis. Unfortunately, not all …

Serial femtosecond crystallography was initially developed for room-temperature X-ray
diffraction studies of macromolecules at X-ray free electron lasers. When combined with …

The main protease (Mpro) of SARS-CoV-2 is critical for viral function and a key drug target.
Mpro is only active when reduced; turnover ceases upon oxidation but is restored by re …

Droplet injection strategies are a promising tool to reduce the large amount of sample
consumed in serial femtosecond crystallography (SFX) measurements at X-ray free electron …

Time-resolved crystallography enables the visualization of protein molecular motion during
a reaction. Although light is often used to initiate reactions in time-resolved crystallography …

The understanding of signal transduction mechanisms in photoreceptor proteins is essential
for elucidating how living organisms respond to light as environmental stimuli. In this study …

X-ray free-electron lasers (XFEL) provide unique opportunities for hard X-ray emission
spectroscopy (XES) in solution phase by enabling femtosecond temporal resolution. The …

Pump–probe experiments at X-ray free-electron laser (XFEL) facilities are a powerful tool for
studying dynamics at ultrafast and longer timescales. Observing the dynamics in diverse …