Viral carrier transport efficiency of gene delivery is high, depending on the type of vector.
However, viral delivery poses significant safety concerns such as inefficient/unpredictable …

Tissue nanotransfection (TNT) is an electromotive gene transfer technology that was
developed to achieve tissue reprogramming in vivo. This protocol describes how to fabricate …

Viral vectors represent a bottleneck in the manufacturing of cellular therapies.
Electroporation has emerged as an approach for non-viral transfection of primary cells, but …

On-target integration of large cassettes via homology-directed repair (HDR) has several
applications. However, the HDR-mediated targeted knock-in suffered from low efficiency. In …

The exact mechanisms of nucleic acid (NA) delivery with gene electrotransfer (GET) are still
unknown, which represents a limitation for its broader use. Further, not knowing the effects …

Abstract Background Cytotoxic T lymphocytes (CTLs) are central players in the adaptive
immune response. Their functional characterization and clinical research depend on …

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and
somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm …

Long-term nondestructive monitoring of cells is of significant importance for understanding
cell proliferation, cell signaling, cell death, and other processes. However, traditional …

This study aims to explore the stability of lipo-polymeric niosomes/niosome-based pCMS-
EGFP complexes under different storage temperatures (25° C, 4° C, and− 20° C). To date …

Maternal germ plasm determines the germline in birds. Previously, we proposed the chicken-
specific Bucky ball (cBuc) as a functional equivalent of the zebrafish germ plasm organizer …