Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
Advanced point‐of‐care testing technologies for human acute respiratory virus detection
The ever‐growing global threats to human life caused by the human acute respiratory virus
(RV) infections have cost billions of lives, created a significant economic burden, and …
(RV) infections have cost billions of lives, created a significant economic burden, and …
Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …
Review of analytical performance of COVID-19 detection methods
In the recent SARS-CoV-2 pandemic, public health experts have emphasized testing,
tracking infected people, and tracing their contacts as an effective strategy to reduce the …
tracking infected people, and tracing their contacts as an effective strategy to reduce the …
CRISPR-Cas based virus detection: Recent advances and perspectives
L Yin, S Man, S Ye, G Liu, L Ma - Biosensors and Bioelectronics, 2021 - Elsevier
Viral infections are one of the most intimidating threats to human beings. One convincing
example is the coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2. Rapid …
example is the coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2. Rapid …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
Droplet Cas12a assay enables DNA quantification from unamplified samples at the single-molecule level
DNA quantification is important for biomedical research, but the routinely used techniques
rely on nucleic acid amplification which have inherent issues like cross-contamination risk …
rely on nucleic acid amplification which have inherent issues like cross-contamination risk …
CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety
Z Mao, R Chen, X Wang, Z Zhou, Y Peng, S Li… - Trends in Food Science …, 2022 - Elsevier
Background In the context of the current pandemic caused by the novel coronavirus,
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
Factors influencing the antimicrobial mechanism of chitosan action and its derivatives: A review
Chitosan, a versatile amino polysaccharide biopolymer derived from chitin, exhibits broad-
spectrum antimicrobial activity against various pathogenic microorganisms, including gram …
spectrum antimicrobial activity against various pathogenic microorganisms, including gram …