Innovative immunization strategies for antivenom development

E Bermúdez-Méndez, A Fuglsang-Madsen, S Føns… - Toxins, 2018 - mdpi.com
Snakes, scorpions, and spiders are venomous animals that pose a threat to human health,
and severe envenomings from the bites or stings of these animals must be treated with …

[HTML][HTML] Strategies for heterologous expression, synthesis, and purification of animal venom toxins

E Rivera-de-Torre, C Rimbault, TP Jenkins… - … in bioengineering and …, 2022 - frontiersin.org
Animal venoms are complex mixtures containing peptides and proteins known as toxins,
which are responsible for the deleterious effect of envenomations. Across the animal …

Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species

G de La Rosa, F Olvera, IG Archundia… - Nature …, 2019 - nature.com
Antivenoms are fundamental in the therapy for snakebites. In elapid venoms, there are
toxins, eg short-chain α-neurotoxins, which are quite abundant, highly toxic, and …

Venom biotechnology: casting light on nature's deadliest weapons using synthetic biology

T Lüddecke, A Paas, RJ Harris, L Talmann… - … in bioengineering and …, 2023 - frontiersin.org
Venoms are complex chemical arsenals that have evolved independently many times in the
animal kingdom. Venoms have attracted the interest of researchers because they are an …

Short-chain consensus alpha-neurotoxin: a synthetic 60-mer peptide with generic traits and enhanced immunogenic properties

G de la Rosa, LL Corrales-García, X Rodriguez-Ruiz… - Amino Acids, 2018 - Springer
The three-fingered toxin family and more precisely short-chain α-neurotoxins (also known as
Type I α-neurotoxins) are crucial in defining the elapid envenomation process, but …

Cloning and sequencing of three-finger toxins from the venom glands of four Micrurus species from Mexico and heterologous expression of an alpha-neurotoxin from …

JF Guerrero-Garzón, M Bénard-Valle… - Biochimie, 2018 - Elsevier
The three-finger toxins (3FTxs) represent an extremely diverse protein family in elapid
venoms, where the short chain α-neurotoxins are the most relevant toxin group from the …

cDNA Cloning, Heterologous Expression, Cytotoxicity, and Inhibitory Effects of a Disintegrin from Bothrops ammodytoides Venom

H Clement, LL Corrales-García… - International Journal of …, 2023 - Springer
An mRNA transcript that codes for a Disintegrin and a Metalloprotease from a venom gland
of the viper Bothrops ammodytoides was isolated. Vector pCR® 2.1-TOPO was used to …

Antibacterial activity and phospholipid recognition of the recombinant defensin J1-1 from Capsicum genus

F Guillén-Chable, I Arenas-Sosa, I Islas-Flores… - Protein Expression and …, 2017 - Elsevier
The gene of the four disulfide-bridged defensin J1-1 from Capsicum was cloned into the
expression vector pQE30 containing a 6His-tag as fusion protein. This construct was …

Antibodies against a single fraction of Micrurus dumerilii venom neutralize the lethal effect of whole venom

J Gómez-Robles, P Rey-Suárez, JA Pereañez… - Toxicology Letters, 2023 - Elsevier
Abstract The coralsnake Micrurus dumerilii (Elapidae) is reported to cause envenomings of
medical importance. Previous studies characterized the protein composition of its venom …

Heterologous Expression and Immunogenic Potential of the Most Abundant Phospholipase A2 from Coral Snake Micrurus dumerilii to Develop Antivenoms

LE Romero-Giraldo, S Pulido, MA Berrío, MF Flórez… - Toxins, 2022 - mdpi.com
Micrurus dumerilii is a coral snake of clinic interest in Colombia. Its venom is mainly
composed of phospholipases A2 being MdumPLA2 the most abundant protein …