The role of fluctuations in protein thermostability has recently received considerable
attention. In the current literature a dualistic picture can be found: thermostability seems to …

The use of enzymes for industrial and biomedical applications is limited to their function at
elevated temperatures. The principles of thermostability engineering need to be …

Non-covalent interactions are weak interactions between atoms or molecules where no
chemical reaction takes place. Because no formation or breaking of chemical bonds is …

Improvement in protein thermostability was often found to be associated with increase in its
proteolytic resistance as revealed by comparative studies of homologous proteins from …

Cells steadily adapt their membrane glycerophospholipid (GPL) composition to changing
environmental and developmental conditions. While the regulation of membrane …

Thermophilic l‐asparaginases display high stability and activity at elevated temperatures.
However, they are of limited use in leukemia therapy because of their low substrate affinity …

Improving the thermostability of industrial enzymes is an important protein engineering
challenge. Point mutations, induced to increase thermostability, affect the structure and …

The adaptation of microorganisms to extreme living temperatures requires the evolution of
enzymes with a high catalytic efficiency under these conditions. Such extremophilic …

Most life forms on earth live at temperatures below 50° C. Within these organisms are
proteins that form the three-dimensional structures essential to their biological activity and …

Starch-based industries require a robust alpha-amylase capable of withstanding elevated
temperatures and long incubation periods. In this study, a loop-grafting strategy based on a …