It has been over four decades since the development of monoclonal antibodies (mAbs)
using a hybridoma cell line was first reported. Since then more than thirty therapeutic …

The role in human health of therapeutic proteins in general, and monoclonal antibodies
(mAbs) in particular, has been significant and is continuously evolving. A considerable …

Over the last two decades, nanobodies or single-domain antibodies have found their way in
research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid …

Increasing attention has been paid to developability assessment with the understanding that
thorough evaluation of monoclonal antibody lead candidates at an early stage can avoid …

Recombinant monoclonal antibodies (mAbs) intended for therapeutic usage are required to
be thoroughly characterized, which has promoted an extensive effort towards the …

Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has within the last 25 years
evolved to become a well-established technique for analysing the solution-phase …

Differential hydrogen exchange-mass spectrometry (HX-MS) measurements are valuable for
identification of differences in the higher order structures of proteins. Typically, the data sets …

We examined how polysorbate 20 (PS20; Tween 20) and polysorbate 80 (PS80; Tween 80)
affect the higher-order structure of a monoclonal antibody (mAb) and its antigen-binding …

Proteins are composed exclusively of l-amino acids. Among elderly individuals, however, d-
aspartic acid (d-Asp) residues have been found in eye lens and brain, as well as in other …

Since the first approval of the anti-CD3 recombinant monoclonal antibody (mAb),
muromonab-CD3, a mouse antibody for the prevention of transplant rejection, by the US …