Advances in cryopreservation of bull sperm

MR Ugur, A Saber Abdelrahman, HC Evans… - Frontiers in veterinary …, 2019 - frontiersin.org
Cryopreservation of semen and artificial insemination have an important, positive impact on
cattle production, and product quality. Through the use of cryopreserved semen and artificial …

Mysteries and unsolved problems of mammalian fertilization and related topics

R Yanagimachi - Biology of reproduction, 2022 - academic.oup.com
Mammalian fertilization is a fascinating process that leads to the formation of a new
individual. Eggs and sperm are complex cells that must meet at the appropriate time and …

Extending resolution within a single imaging frame

E Torres-García, R Pinto-Cámara, A Linares… - Nature …, 2022 - nature.com
The resolution of fluorescence microscopy images is limited by the physical properties of
light. In the last decade, numerous super-resolution microscopy (SRM) approaches have …

Role of calcium oscillations in sperm physiology

E Mata-Martínez, C Sánchez-Cárdenas, JC Chávez… - Biosystems, 2021 - Elsevier
Intracellular Ca 2+ is a key regulator of cell signaling and sperm are not the exception. Cells
often use cytoplasmic Ca 2+ concentration ([Ca 2+] i) oscillations as a means to decodify …

Sperm differentiation: the role of trafficking of proteins

ME Teves, ERS Roldan, D Krapf, JF Strauss III… - International journal of …, 2020 - mdpi.com
Sperm differentiation encompasses a complex sequence of morphological changes that
takes place in the seminiferous epithelium. In this process, haploid round spermatids …

Discrete dynamic model of the mammalian sperm acrosome reaction: the influence of acrosomal pH and physiological heterogeneity

A Aldana, J Carneiro, G Martínez-Mekler… - Frontiers in …, 2021 - frontiersin.org
The acrosome reaction (AR) is an exocytotic process essential for mammalian fertilization. It
involves diverse physiological changes (biochemical, biophysical, and morphological) that …

Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Bos …

R Zhang, C Liang, X Guo, P Bao, J Pei, F Wu… - Frontiers in …, 2022 - frontiersin.org
Mammalian spermatozoa are not mature after ejaculation and must undergo additional
functional and structural changes within female reproductive tracts to achieve subsequent …

Enhanced expansion and reduced kiss-and-run events in fusion pores steered by synaptotagmin-1 C2B domains

AL Di Bartolo, CN Tomes, LS Mayorga… - Journal of Chemical …, 2022 - ACS Publications
The fusion pore controls the release of exocytotic vesicle contents through a precise
orchestration of lipids from the fusing membranes and proteins. There is a major lipid …

Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time

PA Balestrini, M Jabloñski… - Molecular …, 2020 - Wiley Online Library
Acrosomal exocytosis (AR) is a critical process that sperm need to undergo to fertilize an
egg. The evaluation of the presence or absence of the acrosome is usually performed by …

Cryo-EM Structure of Actin Filaments from Zea mays Pollen

Z Ren, Y Zhang, Y Zhang, Y He, P Du, Z Wang… - The Plant …, 2019 - academic.oup.com
Actins are among the most abundant and conserved proteins in eukaryotic cells, where they
form filamentous structures that perform vital roles in key cellular processes. Although large …