Evolution of plant metabolism: the state-of-the-art

AR Fernie, S de Vries… - Philosophical …, 2024 - royalsocietypublishing.org
Immense chemical diversity is one of the hallmark features of plants. This chemo-diversity is
mainly underpinned by a highly complex and biodiverse biochemical machinery. Plant …

Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway

W Ma, M Liu, Z Zhang, Y Xu, P Huang, D Guo… - Communications …, 2022 - nature.com
Presently, the supply of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)
traditionally produced by marine fisheries will be insufficient to meet their market demand in …

Exploring the onset of B12‐based mutualisms using a recently evolved Chlamydomonas auxotroph and B12‐producing bacteria

F Bunbury, E Deery, AP Sayer… - Environmental …, 2022 - Wiley Online Library
Cobalamin (vitamin B12) is a cofactor for essential metabolic reactions in multiple eukaryotic
taxa, including major primary producers such as algae, and yet only prokaryotes can …

Quantitative proteomics insights into Chlamydomonas reinhardtii thermal tolerance enhancement by a mutualistic interaction with Sinorhizobium meliloti

N Zhao, F Liu, W Dong, J Yu, LJ Halverson… - Microbiology …, 2024 - journals.asm.org
Interactions between photosynthetic microalgae and bacteria impact the physiology of both
partners, which influence the fitness and ecological trajectories of each partner in an …

The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing

RA Freudenberg, L Wittemeier, A Einhaus, T Baier… - Cells, 2022 - mdpi.com
Biotechnological application of the green microalga Chlamydomonas reinhardtii hinges on
the availability of selectable markers for effective expression of multiple transgenes …

Utilizing high‐resolution ribosome profiling for the global investigation of gene expression in Chlamydomonas

VL Gotsmann, MKY Ting, N Haase, S Rudorf… - The Plant …, 2024 - Wiley Online Library
Ribosome profiling (Ribo‐seq) is a powerful method for the deep analysis of translation
mechanisms and regulatory circuits during gene expression. Extraction and sequencing of …

Conserved cobalamin acquisition protein 1 is essential for vitamin B12 uptake in both Chlamydomonas and Phaeodactylum

AP Sayer, M Llavero-Pasquina, K Geisler… - Plant …, 2024 - academic.oup.com
Microalgae play an essential role in global net primary productivity and global
biogeochemical cycling. Despite their phototrophic lifestyle, over half of algal species …

Regulation of nucleus‐encoded trans‐acting factors allows orthogonal fine‐tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii

PM Mordaka, K Clouston… - Plant Biotechnology …, 2024 - Wiley Online Library
The green microalga Chlamydomonas reinhardtii is a promising host organism for the
production of valuable compounds. Engineering the Chlamydomonas chloroplast genome …

Evidence for corrin biosynthesis in the last universal common ancestor

LD Modjewski, V Karavaeva, N Mrnjavac… - The FEBS …, 2024 - Wiley Online Library
Corrinoids are cobalt‐containing tetrapyrroles. They include adenosylcobalamin (vitamin
B12) and cobamides that function as cofactors and coenzymes for methyl transfer, radical …

Presence of vitamin B12 metabolism in the last common ancestor of land plants

RG Dorrell, C Nef, S Altan-Ochir… - Philosophical …, 2024 - royalsocietypublishing.org
Vitamin B12, also known as cobalamin, is an essential organic cofactor for methionine
synthase (METH), and is only synthesized by a subset of bacteria. Plants and fungi have an …