Protein–polyelectrolyte interactions

AB Kayitmazer, D Seeman, BB Minsky, PL Dubin, Y Xu - Soft Matter, 2013 - pubs.rsc.org
The interactions of proteins and polyelectrolytes lead to diverse applications in separations,
delivery and wound repair, and are thus of interest to scientists in eg (a) glycobiology,(b) …

Recovery and purification of plant-made recombinant proteins

LR Wilken, ZL Nikolov - Biotechnology advances, 2012 - Elsevier
Plants are becoming commercially acceptable for recombinant protein production for human
therapeutics, vaccine antigens, industrial enzymes, and nutraceuticals. Recently, significant …

Protein purification by polyelectrolyte coacervation: influence of protein charge anisotropy on selectivity

Y Xu, M Mazzawi, K Chen, L Sun, PL Dubin - Biomacromolecules, 2011 - ACS Publications
The effect of polyelectrolyte binding affinity on selective coacervation of proteins with the
cationic polyelectrolyte, poly (diallyldimethylammonium chloride)(PDADMAC), was …

Roles of cigar microbes in flavor formation during roasted-rice leachate fermentation

X Fang, Y Qin, T Liu, S Guo, C Wu, R Zhang… - Applied Microbiology …, 2024 - Springer
Roasted-rice leachate fermentation, a distinctive local tobacco fermentation method in
Sichuan, imparts a mellow flavor and glossy texture to tobacco leaves, along with a roasted …

Aggregative protein–polyelectrolyte complex for high-concentration formulation of protein drugs

T Kurinomaru, K Shiraki - International journal of biological …, 2017 - Elsevier
Aggregative protein–polyelectrolyte complex (PPC) has been proposed as a concentrated
state of protein with a great potential for biopharmaceutical application. In this review article …

Protein partitioning in poly (ethylene glycol)/sodium polyacrylate aqueous two-phase systems

HO Johansson, FM Magaldi, E Feitosa… - … of chromatography A, 2008 - Elsevier
The partition of hemoglobin, lysozyme and glucose-6-phospate dehydrogenase (G6PDH) in
a novel inexpensive aqueous two-phase system (ATPS) composed by poly (ethylene …

Selective protein complexation and coacervation by polyelectrolytes

Y Xu, M Liu, M Faisal, Y Si, Y Guo - Advances in Colloid and Interface …, 2017 - Elsevier
This review discusses the possible relationship between protein charge anisotropy, protein
binding affinity, polymer structure, and selective phase separation. We hope that a …

A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either …

P Opdensteinen, A Lobanov, JF Buyel - Biotechnology Journal, 2021 - Wiley Online Library
Incubation at pH 4.0 or blanching at∼ 65° C facilitates the purification of biopharmaceutical
proteins from plants by precipitating most of the host cell proteins (HCPs) before …

The extraction of tobacco protein from discarded tobacco leaf by hollow fiber membrane integrated process

W Shi, H Li, X Zeng, H Zhang, X Qin - Innovative Food Science & Emerging …, 2019 - Elsevier
Plant leaf protein, especially soluble protein in tobacco leaves, has high nutritional and
medicinal value, and has been gradually applied in the food field as an important source of …

Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein

S Robert, MC Goulet, MA D'Aoust… - Plant Biotechnology …, 2015 - Wiley Online Library
A key factor influencing the yield of biopharmaceuticals in plants is the ratio of recombinant
to host proteins in crude extracts. Postextraction procedures have been devised to enrich …