The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …

Repeated DNA makes up a large fraction of a typical mammalian genome, and some
repetitive elements are able to move within the genome (transposons and retrotransposons) …

Following Cas9 cleavage, DNA repair without a donor template is generally considered
stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that …

The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants
is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases …

We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …

Although base editors are widely used to install targeted point mutations, the factors that
determine base editing outcomes are not well understood. We characterized sequence …

Macroautophagy is an intracellular degradation system that utilizes the autophagosome to
deliver cytoplasmic components to the lysosome. Measuring autophagic activity is critically …

During collective migration of epithelial cells, the migration direction is aligned over a tissue-
scale expanse. Although the collective cell migration is known to be directed by mechanical …

The eye lens of vertebrates is composed of fibre cells in which all membrane-bound
organelles undergo degradation during terminal differentiation to form an organelle-free …

Sprouting angiogenesis requires the coordinated behaviour of endothelial cells, regulated
by Notch and vascular endothelial growth factor receptor (VEGFR) signalling. Here, we use …