Protocells: Milestones and recent advances

I Gözen, ES Köksal, I Põldsalu, L Xue, K Spustova… - Small, 2022 - Wiley Online Library
The origin of life is still one of humankind's great mysteries. At the transition between
nonliving and living matter, protocells, initially featureless aggregates of abiotic matter, gain …

[HTML][HTML] Recent advances in using liposomes for delivery of nucleic acid-based therapeutics

H Nsairat, W Alshaer, F Odeh, E Esawi, D Khater… - OpenNano, 2023 - Elsevier
Nucleic acid therapeutics are rapidly expanding because of recent advancements in
production and purification. This class of therapeutics may change the field of disease …

A short peptide synthon for liquid–liquid phase separation

M Abbas, WP Lipiński, KK Nakashima, WTS Huck… - Nature Chemistry, 2021 - nature.com
Liquid–liquid phase separation of disordered proteins has emerged as a ubiquitous route to
membraneless compartments in living cells, and similar coacervates may have played a role …

Small-molecule organocatalysis facilitates in situ nucleotide activation and RNA copying

HRM Aitken, TH Wright, A Radakovic… - Journal of the …, 2023 - ACS Publications
A key challenge in origin-of-life research is the identification of plausible conditions that
facilitate multiple steps along the pathway from chemistry to biology. The incompatibility of …

Optimization of the inverted emulsion method for high‐yield production of biomimetic giant unilamellar vesicles

A Moga, N Yandrapalli, R Dimova… - ChemBioChem, 2019 - Wiley Online Library
In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the
construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's …

Exploring giant unilamellar vesicle production for artificial cells—Current challenges and future directions

L Van de Cauter, L van Buren, GH Koenderink… - Small …, 2023 - Wiley Online Library
Creating an artificial cell from the bottom up is a long‐standing challenge and, while
significant progress has been made, the full realization of this goal remains elusive …

Optimized cDICE for efficient reconstitution of biological systems in giant unilamellar vesicles

L Van de Cauter, F Fanalista, L Van Buren… - ACS Synthetic …, 2021 - ACS Publications
Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in
reconstitution experiments. They are also widely used in research on synthetic cells, as they …

Enhancing the preservation of liposomes: The role of cryoprotectants, lipid formulations and freezing approaches

F Susa, G Bucca, T Limongi, V Cauda, R Pisano - Cryobiology, 2021 - Elsevier
In the last decades, liposomes acquired a striking success in the biomedical field thanks to
their biocompatibility and drug delivery ability. Many liposomal drug formulations have been …

Determining the “biosignature threshold” for life detection on biotic, abiotic, or prebiotic worlds

LM Barge, LE Rodriguez, JM Weber, BP Theiling - Astrobiology, 2022 - liebertpub.com
The field of prebiotic chemistry has demonstrated that complex organic chemical systems
that exhibit various life-like properties can be produced abiotically in the laboratory …

Microfluidic handling and analysis of giant vesicles for use as artificial cells: a review

T Robinson - Advanced Biosystems, 2019 - Wiley Online Library
One of the goals of synthetic biology is the bottom‐up construction of an artificial cell, the
successful realization of which could shed light on how cellular life emerged and could also …