Compartmentalizing cell-free systems: toward creating life-like artificial cells and beyond

E Cho, Y Lu - ACS Synthetic Biology, 2020 - ACS Publications
Building an artificial cell is a research area that is rigorously studied in the field of synthetic
biology. It has brought about much attention with the aim of ultimately constructing a natural …

Advances in cell‐free biosensors: principle, mechanism, and applications

L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …

Electrochemical cell‐free biosensors for antibody detection

S Bracaglia, S Ranallo, F Ricci - Angewandte Chemie, 2023 - Wiley Online Library
We report here the development of an electrochemical cell‐free biosensor for antibody
detection directly in complex sample matrices with high sensitivity and specificity that is …

Cell-free gene expression: methods and applications

AC Hunt, BJ Rasor, K Seki, HM Ekas, KF Warfel… - Chemical …, 2024 - ACS Publications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Recent advances in design of fluorescence-based assays for high-throughput screening

X Fang, Y Zheng, Y Duan, Y Liu, W Zhong - Analytical chemistry, 2018 - ACS Publications
Significant improvements in disease management have been achieved in the past few
decades. Using cancer as an example, survival rates have increased dramatically in …

Translating new synthetic biology advances for biosensing into the earth and environmental sciences

I Del Valle, EM Fulk, P Kalvapalle, JJ Silberg… - Frontiers in …, 2021 - frontiersin.org
The rapid diversification of synthetic biology tools holds promise in making some classically
hard-to-solve environmental problems tractable. Here we review longstanding problems in …

Enzyme-assisted nucleic acid detection for infectious disease diagnostics: moving toward the point-of-care

A Suea-Ngam, L Bezinge, B Mateescu, PD Howes… - ACS …, 2020 - ACS Publications
Driven by complex and interconnected factors, including population growth, climate change,
and geopolitics, infectious diseases represent one of the greatest healthcare challenges of …

Cell-free biomanufacturing

BC Bundy, JP Hunt, MC Jewett, JR Swartz… - Current opinion in …, 2018 - Elsevier
Since its early development and use to decipher the genetic code, in vitro or 'cell-
free'systems have been used as an important research tool to understand biochemical …

Reengineering cell-free protein synthesis as a biosensor: Biosensing with transcription, translation, and protein-folding

M Soltani, BR Davis, H Ford, JAD Nelson… - Biochemical Engineering …, 2018 - Elsevier
Biosensors highjack and reengineer biological systems for highly specific detection of
diverse molecules. However, many traditional biosensor devices have slow response times …

Cell-free protein synthesis system for bioanalysis: Advances in methods and applications

Y Gu, F Fan, Y Liu, Y Chai, Y Yuan, X Chen - TrAC Trends in Analytical …, 2023 - Elsevier
Efficient, dynamic and accurate are the iterative trends of modern bioanalysis. Current cell-
based platforms are hindered by time-consuming cell culture cycles, physiological …