Determining the structure and mechanisms of all individual functional modules of cells at
high molecular detail has often been seen as equal to understanding how cells work …

Single-molecule localization microscopy (SMLM) enables imaging scientists to visualize
biological structures with unprecedented resolution. Particularly powerful implementations of …

Quantitative data analysis is important for any single-molecule localization microscopy
(SMLM) workflow to extract biological insights from the coordinates of the single …

Clathrin forms a triskelion, or three-legged, network that regulates cellular processes by
facilitating cargo internalization and trafficking in eukaryotes. Its N-terminal domain is crucial …

Liquid–liquid phase separation is a major mechanism for organizing macromolecules,
particularly proteins with intrinsically disordered regions, in compartments not limited by a …

In recent years, notable progress has been achieved in both the hardware and algorithms of
structured illumination microscopy (SIM). Nevertheless, the advancement of three …

Modern microscopy relies increasingly on microscope automation to improve throughput,
ensure reproducibility or observe rare events. Automation requires computer control of the …

Membrane budding, which underlies fundamental processes like endocytosis, intracellular
trafficking, and viral infection, is thought to involve membrane coat-forming proteins …

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular
localization tools to study molecular structure at the nanoscale level in the intact cell …

The synaptonemal complex (SC) is a zipper-like protein assembly that links homologous
chromosomes to regulate recombination and segregation during meiosis. The SC has been …