Expression and function of odorant receptors (ORs), which account for more than 50% of G
protein–coupled receptors, are being increasingly reported in nonolfactory sites. However …

The high-throughput antibiotic resistance gene (ARG) qPCR array, initially published in
2012, is increasingly used to quantify resistance and mobile determinants in environmental …

Computational tool-assisted primer design for real-time reverse transcription (RT) PCR
(qPCR) analysis largely ignores the sequence similarities between sequences of …

Real-time quantitative polymerase chain reaction (qPCR) is one of the most important
methods for analyzing the expression patterns of target genes. However, successful qPCR …

Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used
routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR …

Резюме За три десятилетия своего существования полимеразная цепная реакция
(ПЦР) стала практически вездесущей. Приблизительно в каждой восьмой работе, где …

Various harmful microalgae coexist in the marine environment, seriously affecting the
survival of aquatic animals, the sustainable development of fisheries, and pose a great …

Microglia (MG), the principal neuroimmune sentinels in the brain, continuously sense
changes in their environment and respond to invading pathogens, toxins, and cellular …

Odorant receptors are the largest subfamily of G protein-coupled receptors and were
recently suggested to play critical roles in nonolfactory tissues. However, the expression and …

Real-time quantitative polymerase chain reaction (qPCR) is a powerful tool for analyzing
and quantifying gene expression, and its primer design is the first and most important step …