NOTE: In the version of this Review initially published, an author (B. Martin Hallberg) was left
off of the author list. This information has been added to the HTML and PDF versions of the …

Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the
predictable modification of genomes as they enable precise genome editing in heterologous …

As a result of numerous genome sequencing projects, large numbers of candidate open
reading frames are being identified, many of which have no known function. Analysis of …

Genome engineering techniques represented by the Cre–loxP recombination system have
been used extensively for biomedical research. However, powerful and useful techniques …

▪ Abstract Structural models of site-specific recombinases from the lambda integrase family
of enzymes have in the last four years provided an important new perspective on the three …

The Cre-lox system is often used to manipulate sequences in mammalian genomes. We
have observed that continuous expression of the Cre recombinase in cultured cells lacking …

The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

The geometry of the phosphodiester backbone was analyzed for 7739 dinucleotides from
447 selected crystal structures of naked and complexed DNA. Ten torsion angles of a near …

Cre recombinase from bacteriophage P1 recognizes a 34-bp recombination site, loxP, with
exquisite sequence specificity and catalyzes the site-specific insertion, excision, or …

Structural studies of protein-DNA complexes have shown that there are many distinct
families of DNA-binding proteins, and have shown that there is no simple “code” describing …