This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …

Housekeeping genes are involved in basic cell maintenance and, therefore, are expected to
maintain constant expression levels in all cells and conditions. Identification of these genes …

Single-cell RNA sequencing (scRNA-seq) technologies are poised to reshape the current
cell-type classification system. However, a transcriptome-based single-cell atlas has not …

Zika virus (ZIKV) is an arbovirus belonging to the genus Flavivirus (family Flaviviridae) and
was first described in 1947 in Uganda following blood analyses of sentinel Rhesus …

Abstract Type 2 diabetes (T2D) is characterized by chronic hyperglycemia resulting from a
deficiency in insulin signaling, because of insulin resistance and/or defects in insulin …

The cohesin complex has an essential role in maintaining genome organization. However,
its role in gene regulation remains largely unresolved. Here we report that the cohesin …

Housekeeping (HK) genes are constitutively expressed genes that are required for the
maintenance of basic cellular functions. Despite their importance in the calibration of gene …

The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq
is likely to become the platform of choice for interrogating steady state RNA. In order to …

Ultra-high-throughput sequencing is emerging as an attractive alternative to microarrays for
genotyping, analysis of methylation patterns, and identification of transcription factor binding …

Promiscuous gene expression (PGE) by thymic epithelial cells (TEC) is essential for
generating a diverse T cell antigen receptor repertoire tolerant to self-antigens, and thus for …