The ability to multiplex PCR by probe color and melting temperature (Tm) greatly expands
the power of real-time analysis. Simple hybridization probes with only a single fluorescent …

Quality assurance in the food industry in recent years has involved the acceptance and
implementation of a variety of nucleic acid–based methods for rapid and sensitive detection …

Background: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was
recently introduced as a homogeneous, closed-tube method of genotyping that does not …

Background: Common methods for identification of DNA sequence variants use gel
electrophoresis or column separation after PCR. Methods: We developed a method for …

High-resolution melting was recently introduced as a technique to genotype single-
nucleotide polymorphisms (SNPs) within small amplicons (1). This closed-tube method …

The LightCycler® is a real-time PCR instrument that combines a thermocycler and a micro-
volume fluorimeter. LightCycler technology is gaining popularity due to its ability to detect …

LightCycler technology combines rapid-cycle polymerase chain reaction with real-time
fluorescent monitoring and melting curve analysis. Since its introduction in 1997, it is now …

The invention provides methods and devices for analyzing sequence variations in nucleic
acid samples comprising multiple loci, each having tWo, three or more possible allelic …

Aims To understand more clearly the genetic ontogeny of inverted papilloma of urinary
bladder, we analysed telomerase reverse transcriptase (TERT) promoter mutation status in a …

Over the past 5 years, there has been substantial progress in sequencing the human
genome and identifying clinically significant genes (1). Genes that are clinically significant …