Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references

S Guenin, M Mauriat, J Pelloux… - Journal of …, 2009 - academic.oup.com
Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-
PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses …

Achievements and challenges in legume breeding for pest and disease resistance

D Rubiales, S Fondevilla, W Chen… - Critical Reviews in …, 2015 - Taylor & Francis
Yield stability of legume crops is constrained by a number of pest and diseases. Major
diseases are rusts, powdery and downy mildews, ascochyta blights, botrytis gray molds …

Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering

JY Zhang, MH Cruz De Carvalho… - Plant, cell & …, 2014 - Wiley Online Library
M edicago truncatula is a model legume forage crop native to the arid and semi‐arid
environments of the Mediterranean. Given its drought‐adapted nature, it is an ideal …

Medicago truncatula IPD3 Is a Member of the Common Symbiotic Signaling Pathway Required for Rhizobial and Mycorrhizal Symbioses

B Horváth, LH Yeun, Á Domonkos… - Molecular plant …, 2011 - Am Phytopath Society
Legumes form endosymbiotic associations with nitrogen-fixing bacteria and arbuscular
mycorrhizal (AM) fungi which facilitate nutrient uptake. Both symbiotic interactions require a …

High phosphate reduces host ability to develop arbuscular mycorrhizal symbiosis without affecting root calcium spiking responses to the fungus

C Balzergue, M Chabaud, DG Barker… - Frontiers in plant …, 2013 - frontiersin.org
The arbuscular mycorrhizal symbiosis associates soil fungi with the roots of the majority of
plants species and represents a major source of soil phosphorus acquisition. Mycorrhizal …

Translating Medicago truncatula genomics to crop legumes

ND Young, M Udvardi - Current opinion in plant biology, 2009 - Elsevier
Genomic resources developed in the model legume, Medicago truncatula, have the
potential to accelerate practical advances in crop legumes. M. truncatula is closely related to …

Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions

JV Die, B Román, S Nadal, CI González-Verdejo - Planta, 2010 - Springer
Reverse transcription quantitative real-time polymerase chain reaction is the most accurate
measure of gene expression in biological systems. The data are analyzed through a process …

Genome-Wide Identification of Medicago Peptides Involved in Macronutrient Responses and Nodulation

TC de Bang, PK Lundquist, X Dai, C Boschiero… - Plant …, 2017 - academic.oup.com
Growing evidence indicates that small, secreted peptides (SSPs) play critical roles in
legume growth and development, yet the annotation of SSP-coding genes is far from …

MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula

IS Kryvoruchko, S Sinharoy, I Torres-Jerez… - Plant …, 2016 - academic.oup.com
Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for
sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs …

The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis

F Krajinski, PE Courty, D Sieh, P Franken… - The Plant …, 2014 - academic.oup.com
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the
host plant via the mycorrhizal pathway, ie, the fungal uptake of Pi from the soil and its …