Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Optimization of biological networks is often limited by wet lab labor and cost, and the lack of
convenient computational tools. Here, we describe METIS, a versatile active machine …

Cell-free systems for gene expression have gained attention as platforms for the facile study
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …

The past decade has seen the emergence of multidrug resistant pathogens as a leading
cause of death worldwide, reigniting interest in the field of phage therapy. Modern advances …

Streptomyces-based cell-free expression systems have been developed to meet the
demand for synthetic biology applications. However, protein yields from the previous …

Cell-free expression systems (CFEs) are cutting-edge research tools used in the
investigation of biological phenomena and the engineering of novel biotechnologies. While …

Many bacterial mechanisms for highly specific and sensitive detection of heavy metals and
other hazards have been reengineered to serve as sensors. In some cases, these sensors …

The use of linear DNA templates in cell-free systems promises to accelerate the prototyping
and engineering of synthetic gene circuits. A key challenge is that linear templates are …

The field of cell-free synthetic biology is an emerging branch of engineered biology that
allows for rapid prototyping of biological designs and, in its own right, is becoming a venue …