From genomics to gene therapy: induced pluripotent stem cells meet genome editing

A Hotta, S Yamanaka - Annual review of genetics, 2015 - annualreviews.org
The advent of induced pluripotent stem (iPS) cells has opened up numerous avenues of
opportunity for cell therapy, including the initiation in September 2014 of the first human …

[HTML][HTML] Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration

A Kumar, M Lualdi, GT Lyozin, P Sharma… - Developmental …, 2015 - Elsevier
In the early mouse embryo, a specialized population of extraembryonic visceral endoderm
(VE) cells called the distal VE (DVE) arises at the tip of the egg cylinder stage embryo and …

Improved live-cell PCR method for detection of organophosphates degrading opd genes and applications

H Mali, C Shah, AS Prajapati, S Mesara… - Applied Microbiology …, 2022 - Springer
Organophosphates are becoming an emerging pollutant due to their various applications,
particularly as pesticides. In this study, an improved Colony (Live-cell) PCR method was …

Identification of salt-responsive genes from C4 halophyte Suaeda nudiflora through suppression subtractive hybridization and expression analysis under individual …

S Jothiramshekar, JJ Benjamin, R Krishnasamy… - … and Molecular Biology …, 2020 - Springer
Salinization of soil is a prime abiotic stress that limits agriculture productivity worldwide. To
Study the mechanisms that halophytes take up to survive under high salt condition is …

Live‐cell PCR and one‐step purification streamline DNA engineering

GT Lyozin, L Brunelli - The FASEB Journal, 2020 - Wiley Online Library
In vivo DNA engineering such as recombineering (recombination‐mediated genetic
engineering) and DNA gap repair typically involve growing Escherichia coli (E coli) …

ESI mutagenesis: A one-step method for introducing point mutations into bacterial artificial chromosome transgenes

A Rondelet, A Pozniakovsky, M Leuschner, I Poser… - bioRxiv, 2019 - biorxiv.org
Bacterial artificial chromosome (BAC)-based transgenes have emerged as a powerful tool
for controlled and conditional interrogation of protein function in higher eukaryotes. While …

DNA gap repair in Escherichia coli for multiplex site‐directed mutagenesis

GT Lyozin, L Brunelli - The FASEB Journal, 2020 - Wiley Online Library
Site‐directed mutagenesis allows the generation of novel DNA sequences that can be used
for a variety of important applications such as the functional analysis of genetic variants. To …

Direct isolation of seamless mutant bacterial artificial chromosomes

GT Lyozin, Y Kosaka, G Bhattacharje… - Current Protocols in …, 2017 - Wiley Online Library
Seamless (ie, without unwanted DNA sequences) mutant bacterial artificial chromosomes
(BACs) generated via recombination‐mediated genetic engineering (recombineering) are …

DNA gap repair-mediated site-directed mutagenesis is different from Mandecki and recombineering approaches

GT Lyozin, L Brunelli - BioRxiv, 2018 - biorxiv.org
Site-directed mutagenesis allows the generation of mutant DNA sequences for downstream
functional analysis of genetic variants involved in human health and disease. Understanding …

ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes

A Rondelet, A Pozniakovsky… - Life science …, 2021 - life-science-alliance.org
Bacterial artificial chromosome (BAC)–based transgenes have emerged as a powerful tool
for controlled and conditional interrogation of protein function in higher eukaryotes. Although …