Beyond aggregation: Pathological phase transitions in neurodegenerative disease

C Mathieu, RV Pappu, JP Taylor - Science, 2020 - science.org
Over the past decade, phase transitions have emerged as a fundamental mechanism of
cellular organization. In parallel, a wealth of evidence has accrued indicating that …

[HTML][HTML] Molecular mechanisms of stress granule assembly and disassembly

S Hofmann, N Kedersha, P Anderson… - Biochimica et Biophysica …, 2021 - Elsevier
Stress granules (SGs) are membrane-less ribonucleoprotein (RNP)-based cellular
compartments that form in the cytoplasm of a cell upon exposure to various environmental …

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner

Y Gwon, BA Maxwell, RM Kolaitis, P Zhang, HJ Kim… - Science, 2021 - science.org
INTRODUCTION Stress granules are dynamic structures composed of RNA and protein that
arise in the cytoplasm in response to a variety of stressors. These structures form via liquid …

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles

AM Leidal, HH Huang, T Marsh, T Solvik, D Zhang… - Nature cell …, 2020 - nature.com
Traditionally viewed as an autodigestive pathway, autophagy also facilitates cellular
secretion; however, the mechanisms underlying these processes remain unclear. Here, we …

QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism

Z Zhao, Y Qing, L Dong, L Han, D Wu, Y Li, W Li, J Xue… - Cell, 2023 - cell.com
Summary N 7-methylguanosine (m 7 G) modification, routinely occurring at mRNA 5′ cap
or within tRNAs/rRNAs, also exists internally in messenger RNAs (mRNAs). Although m 7 G …

FUS and TDP-43 phases in health and disease

B Portz, BL Lee, J Shorter - Trends in biochemical sciences, 2021 - cell.com
The distinct prion-like domains (PrLDs) of FUS and TDP-43, modulate phase transitions that
result in condensates with a range of material states. These assemblies are implicated in …

A new phase of networking: the molecular composition and regulatory dynamics of mammalian stress granules

SR Millar, JQ Huang, KJ Schreiber, YC Tsai… - Chemical …, 2023 - ACS Publications
Stress granules (SGs) are cytosolic biomolecular condensates that form in response to
cellular stress. Weak, multivalent interactions between their protein and RNA constituents …

µMap proximity labeling in living cells reveals stress granule disassembly mechanisms

C Pan, SD Knutson, SW Huth… - Nature Chemical …, 2024 - nature.com
Phase-separated condensates are membrane-less intracellular structures comprising
dynamic protein interactions that organize essential biological processes. Understanding …

Ubiquitination is essential for recovery of cellular activities after heat shock

BA Maxwell, Y Gwon, A Mishra, J Peng, H Nakamura… - Science, 2021 - science.org
INTRODUCTION In response to many types of stress, eukaryotic cells initiate an adaptive
and reversible response that includes down-regulation of key cellular activities along with …

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1

DJ Klionsky, AK Abdel-Aziz, S Abdelfatah, M Abdellatif… - autophagy, 2021 - Taylor & Francis
In 2008, we published the first set of guidelines for standardizing research in autophagy.
Since then, this topic has received increasing attention, and many scientists have entered …