Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Optoacoustic (photoacoustic) imaging offers unique opportunities for visualizing biological
function in vivo by achieving high-resolution images of optical contrast much deeper than …

Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be
exploited to create fully genetically encoded and thus subcellularly targetable sensors …

Genetically encoded contrast in photoacoustic imaging (PAI) is complementary to the
intrinsic contrast provided by endogenous absorbing chromophores such as hemoglobin …

Highlights• Genetically encoded biosensors give quantitative readouts of key brain
metabolites.• Biosensors allow investigation of the relationship between activity and …

Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for
quantitative imaging, enabling precise measurement of cellular metabolites, molecular …

FRET is a powerful approach to study the interactions of fluorescent molecules, and
numerous methods have been developed to measure FRET in cells. Here, we present a …

Measurement of Förster resonance energy transfer by fluorescence lifetime imaging
microscopy (FLIM-FRET) is a powerful method for visualization of intracellular signaling …

The steady improvement in the imaging of cellular processes in living tissue over the last 10–
15 years through the use of various fluorophores including organic dyes, fluorescent …

Photoacoustic (PA) imaging is a rapidly emerging biomedical imaging modality that is
capable of visualizing cellular and molecular functions with high detection sensitivity and …