Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Fluorescence nanoscopy in cell biology

SJ Sahl, SW Hell, S Jakobs - Nature reviews Molecular cell biology, 2017 - nature.com
Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging

KKH Chung, Z Zhang, P Kidd, Y Zhang, ND Williams… - Nature …, 2022 - nature.com
DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a
powerful super-resolution microscopy method that can acquire high-fidelity images at …

Single-molecule localization microscopy in eukaryotes

M Sauer, M Heilemann - Chemical reviews, 2017 - ACS Publications
Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine

G Fu, CS Guy, NM Chapman, G Palacios, J Wei… - Nature, 2021 - nature.com
T follicular helper (TFH) cells are crucial for B cell-mediated humoral immunity. Although
transcription factors such as BCL6 drive the differentiation of TFH cells,, it is unclear whether …

Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol

H Kemmoku, K Takahashi, K Mukai, T Mori… - Nature …, 2024 - nature.com
Stimulator of interferon genes (STING) is critical for the type I interferon response to
pathogen-or self-derived DNA in the cytosol. STING may function as a scaffold to activate …

TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulation

JD Worboys, KN Vowell, RK Hare, AR Ambrose… - Nature …, 2023 - nature.com
TIGIT is an inhibitory receptor expressed on lymphocytes and can inhibit T cells by
preventing CD226 co-stimulation through interactions in cis or through competition of shared …

Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination

SV Pageon, T Tabarin, Y Yamamoto… - Proceedings of the …, 2016 - National Acad Sciences
Antigen recognition by the T-cell receptor (TCR) is a hallmark of the adaptive immune
system. When the TCR engages a peptide bound to the restricting major histocompatibility …

Image co-localization–co-occurrence versus correlation

JS Aaron, AB Taylor, TL Chew - Journal of cell science, 2018 - journals.biologists.com
Fluorescence image co-localization analysis is widely utilized to suggest biomolecular
interaction. However, there exists some confusion as to its correct implementation and …