Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects

RJ Gouka, PJ Punt, C Van Den Hondel - Applied microbiology and …, 1997 - Springer
Filamentous fungi are widely used for the production of homologous and heterologous
proteins but, compared to homologous proteins, the levels of production of heterologous …

Genetic manipulation of fungal strains for the improvement of heterologous genes expression (a mini-review)

MM Koushki, H Rouhani, M Farsi - African Journal of Biotechnology, 2011 - ajol.info
Fungi are eukaryotic microorganisms that influence our everyday lives in areas as diverse
as agriculture, medicine and basic science. With the advent of molecular biology, it has been …

Genetically controlled cell lysis in the yeast Saccharomyces cerevisiae

N Zhang, DCJ Gardner, SG Oliver… - Biotechnology and …, 1999 - Wiley Online Library
The cell wall of the yeast Saccharomyces cerevisiae is a tough, rigid structure, which
presents a significant barrier to the release of native or recombinant proteins from this …

Identifying a species-specific region of yeast TFIIB in vivo

SP Shaw, J Wingfield, MJ Dorsey… - Molecular and cellular …, 1996 - Taylor & Francis
The general transcription factor IIB (TFIIB) is required for RNA polymerase II transcription in
eukaryotes. It provides a physical link between the TATA-binding protein (TBP) and the RNA …

A new system for the release of heterologous proteins from yeast based on mutant strains deficient in cell integrity

P Alvarez, M Sampedro, M Molina, C Nombela - Journal of biotechnology, 1994 - Elsevier
A system has been developed for the release of heterologous proteins from Saccharomyces
cerevisiae, based on the use of thermosensitive osmotic-remedial mutants, deficient in cell …

[PDF][PDF] Functional studies of the secretory pathway of filamentous fungi: The effect of unfolded protein response on protein production

M Valkonen - 2003 - researchgate.net
This study describes the cloning and characterisation of three genes from the filamentous
fungus Trichoderma reesei. Snc1 encoding a putative v-SNARE was shown to be the …

[PDF][PDF] Functional studies of the secretory pathway of filamentous fungi

M Valkonen - The effect of unfolded protein response on protein …, 2003 - sarjaweb.vtt.fi
This study describes the cloning and characterisation of three genes from the filamentous
fungus Trichoderma reesei. Snc1 encoding a putative v-SNARE was shown to be the …

[图书][B] Approaches to the study of protein secretion in yeast

B Gill - 1997 - search.proquest.com
Mutants of the yeast Saccharomyces cerevisiae that showed enhanced secretion yield of a
mini-proinsulin reporter protein were isolated, following random mutagenesis, via a footprint …

Method of using aminopeptidase to produce cleaved polypeptides

JC Joly - US Patent 7,632,658, 2009 - Google Patents
(57) ABSTRACT A gram-negative bacterial cell is described that is deficient in a
chromosomal gene present in a wild-type Such cell which gene shares at least 80 …

[引用][C] Production of Cyprosins (plant proteolytic enzymes from

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