Copper (I)-catalyzed 1, 3-dipolar cycloaddition between organic azides and terminal
alkynes, commonly known as CuAAC or click chemistry, has been identified as one of the …

Glycans are involved in numerous biological recognition events. Being secondary gene
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …

Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

Cu (I)-catalyzed azide–alkyne 1, 3-dipolar cycloaddition (CuAAC), popularly known as the
“click reaction”, serves as the most potent and highly dependable tool for facile construction …

Despite advances in genetic and proteomic techniques, a complete portrait of the proteome
and its complement of dynamic interactions and modifications remains a lofty, and as of yet …

Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a
unique monosaccharide modification discovered in the early 1980s. With the technological …

Interrogating biological systems is often limited by access to biological probes. The
emergence of “click chemistry” has revolutionized bioconjugate chemistry by providing facile …

The addition of O-linked-β-DN-acetylglucosamine (O-GlcNAc) onto serine and threonine
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …

Chemical tools have accelerated progress in glycoscience, reducing experimental barriers
to studying protein glycosylation, the most widespread and complex form of posttranslational …

Functional tools are needed to understand complex biological systems. Here we review how
chemical reporters in conjunction with bioorthogonal labeling methods can be used to …