Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Pushing the frontier of fluorescence microscopy requires the design of enhanced
fluorophores with finely tuned properties. We recently discovered that incorporation of four …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Small-molecule fluorophores manifest the ability of chemistry to solve problems in biology.
As we noted in a previous review (Lavis, LD; Raines, RT ACS Chem. Biol. 2008, 3, 142 …

Insufficient brightness of fluorophores poses a major bottleneck for the advancement of
super-resolution microscopes. Despite being widely used, many rhodamine dyes exhibit sub …

A CF3-BODIPY forms strongly luminescent aggregates, which contrasts with the quenched
condensed-phase photophysics that are typical for BODIPY dyes. Examination of the …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

A range of bright and photostable rhodamines and carbopyronines with absorption maxima
in the range of λ= 500–630 nm were prepared, and enabled the specific labeling of …

Small molecule fluorophores are essential tools for chemical biology. A benefit of synthetic
dyes is the ability to employ chemical approaches to control the properties and direct the …