Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Light-sheets and smart microscopy, an exciting future is dawning

S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy

B Yang, M Lange, A Millett-Sikking, X Zhao… - Nature …, 2022 - nature.com
The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

Harnessing non-destructive 3D pathology

JTC Liu, AK Glaser, K Bera, LD True… - Nature biomedical …, 2021 - nature.com
High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Image processing tools for petabyte-scale light sheet microscopy data

X Ruan, M Mueller, G Liu, F Görlitz, TM Fu, DE Milkie… - Nature …, 2024 - nature.com
Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of
subcellular dynamics and large biological specimens. However, it often generates datasets …

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

B Chen, BJ Chang, P Roudot, F Zhou, E Sapoznik… - Nature …, 2022 - nature.com
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity

M Hoffmann, J Henninger, J Veith, L Richter… - Nature …, 2023 - nature.com
Due to the size and opacity of vertebrate brains, it has until now been impossible to
simultaneously record neuronal activity at cellular resolution across the entire adult brain. As …

How it feels in a cell

M Bonucci, T Shu, LJ Holt - Trends in Cell Biology, 2023 - cell.com
Life emerges from thousands of biochemical processes occurring within a shared
intracellular environment. We have gained deep insights from in vitro reconstitution of …

Multiview confocal super-resolution microscopy

Y Wu, X Han, Y Su, M Glidewell, JS Daniels, J Liu… - Nature, 2021 - nature.com
Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …