Super-resolution microscopy: shedding light on the cellular plasma membrane

MB Stone, SA Shelby, SL Veatch - Chemical reviews, 2017 - ACS Publications
Lipids and the membranes they form are fundamental building blocks of cellular life, and
their geometry and chemical properties distinguish membranes from other cellular …

Biological insight from super-resolution microscopy: what we can learn from localization-based images

D Baddeley, J Bewersdorf - Annual review of biochemistry, 2018 - annualreviews.org
Super-resolution optical imaging based on the switching and localization of individual
fluorescent molecules [photoactivated localization microscopy (PALM), stochastic optical …

Defining the rate-limiting processes of bacterial cytokinesis

C Coltharp, J Buss, TM Plumer… - Proceedings of the …, 2016 - National Acad Sciences
Bacterial cytokinesis is accomplished by the essential 'divisome'machinery. The most widely
conserved divisome component, FtsZ, is a tubulin homolog that polymerizes into the 'FtsZ …

One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy

F Fricke, J Beaudouin, R Eils, M Heilemann - Scientific reports, 2015 - nature.com
Probing the oligomeric state of abundant molecules, such as membrane proteins in intact
cells, is essential, but has not been straightforward. We address this challenge with a simple …

In vivo organization of the FtsZ‐ring by ZapA and ZapB revealed by quantitative super‐resolution microscopy

J Buss, C Coltharp, T Huang, C Pohlmeyer… - Molecular …, 2013 - Wiley Online Library
In most bacterial cells, cell division is dependent on the polymerization of the FtsZ protein to
form a ring‐like structure (Z‐ring) at the midcell. Despite its essential role, the molecular …

Unraveling the thousand word picture: an introduction to super-resolution data analysis

A Lee, K Tsekouras, C Calderon, C Bustamante… - Chemical …, 2017 - ACS Publications
Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

A Multi-layered Protein Network Stabilizes the Escherichia coli FtsZ-ring and Modulates Constriction Dynamics

J Buss, C Coltharp, G Shtengel, X Yang, H Hess… - PLoS …, 2015 - journals.plos.org
The prokaryotic tubulin homolog, FtsZ, forms a ring-like structure (FtsZ-ring) at midcell. The
FtsZ-ring establishes the division plane and enables the assembly of the macromolecular …

Stochastic approach to the molecular counting problem in superresolution microscopy

GC Rollins, JY Shin, C Bustamante… - Proceedings of the …, 2015 - National Acad Sciences
Superresolution imaging methods—now widely used to characterize biological structures
below the diffraction limit—are poised to reveal in quantitative detail the stoichiometry of …

Quantitative single-molecule localization microscopy

S Hugelier, PL Colosi… - Annual Review of …, 2023 - annualreviews.org
Super-resolution fluorescence microscopy allows the investigation of cellular structures at
nanoscale resolution using light. Current developments in super-resolution microscopy have …

Model-independent counting of molecules in single-molecule localization microscopy

G Hummer, F Fricke, M Heilemann - Molecular biology of the cell, 2016 - Am Soc Cell Biol
Most biomolecular processes rely on tightly controlled stoichiometries, from the formation of
molecular assemblies to cellular signaling. Single-molecule localization microscopy studies …