Progress in our understanding of several biological processes promises to broaden the
usefulness of Escherichia coli as a tool for gene expression. There is an expanding choice …

Yeasts combine the ease of genetic manipulation and fermentation of a microorganism with
the capability to secrete and modify foreign proteins according to a general eukaryotic …

New textbooks at all levels of chemistry appear with great regularity. Some fields such as
basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil …

Protein aggregation is an ordinary consequence of thermal stress. In recombinant bacteria,
the over-expression of plasmid-encoded genes triggers transcription of heat-shock genes …

Members of the genus Bacillus are widely used as sources of industrial enzymes, fine
biochemicals, antibiotics, and insecticides (for a review, see reference 27). One of these …

Numerous reports have been published over the last decade assessing the potential of
plants as useful hosts for the heterologous expression of clinically useful proteins …

Summary Human Serum Albumin (HSA) accounts for 60% of the total protein in blood serum
and it is the most widely used intravenous protein in a number of human therapies. HSA …

Genetic strategies have been used for more than two decades to improve bacterial
bioprocesses and to simplify recovery procedures. Such strategies include the design of …

In recent years, Escherichia coli gene fusion expression systems have circumvented many
of the problems inherent in the use of this bacterium for the production of recombinant …

Even though secretion offers numerous advantages for the production of proteins in
Escherichia coli, the expression of many heterologous proteins is severely limited by …