Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Simple, efficient and thorough shotgun proteomic analysis with PatternLab V

MDM Santos, DB Lima, JSG Fischer, MA Clasen… - Nature protocols, 2022 - nature.com
Shotgun proteomics aims to identify and quantify the thousands of proteins in complex
mixtures such as cell and tissue lysates and biological fluids. This approach uses liquid …

Search engine processor: filtering and organizing peptide spectrum matches

PC Carvalho, JSG Fischer, T Xu, D Cociorva… - …, 2012 - Wiley Online Library
The search engine processor (SEP ro) is a tool for filtering, organizing, sharing, and
displaying peptide spectrum matches. It employs a novel three‐tier B ayesian approach that …

Proteomics reveals differentially regulated pathways when comparing grade 2 and 4 astrocytomas

DCA Verissimo, AC Camillo-Andrade, MDM Santos… - Plos one, 2023 - journals.plos.org
Astrocytic tumors are known for their high progression capacity and high mortality rates; in
this regard, proteins correlated to prognosis can aid medical conduct. Although several …

PatternLab: from mass spectra to label‐free differential shotgun proteomics

PC Carvalho, JSG Fischer, T Xu… - Current Protocols in …, 2012 - Wiley Online Library
PatternLab for proteomics is a self‐contained computational environment for analyzing
shotgun proteomic data. Recent improvements incorporate modules to facilitate the …

D‐score: a search engine independent MD‐score

M Vaudel, D Breiter, F Beck, J Rahnenführer… - …, 2013 - Wiley Online Library
While peptides carrying PTM s are routinely identified in gel‐free MS, the localization of the
PTM s onto the peptide sequences remains challenging. Search engine scores of secondary …

Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant

DS Chacon, MDM Santos, B Bonilauri… - Frontiers in Plant …, 2022 - frontiersin.org
Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is
widely used in traditional medicine showing anti-inflammatory and central nervous system …

Current methods for global proteome identification

M Vaudel, A Sickmann, L Martens - Expert review of proteomics, 2012 - Taylor & Francis
In a time frame of a few decades, protein identification went from laborious single protein
identification to automated identification of entire proteomes. This shift was enabled by the …

[HTML][HTML] PepExplorer: a similarity-driven tool for analyzing de novo sequencing results

FV Leprevost, RH Valente, DB Lima, J Perales… - Molecular & Cellular …, 2014 - Elsevier
Peptide spectrum matching is the current gold standard for protein identification via mass-
spectrometry-based proteomics. Peptide spectrum matching compares experimental mass …

Corrected and republished from: “Extracellular Vesicle Formation in Cryptococcus deuterogattii Impacts Fungal Virulence”

RF Castelli, A Pereira, L Honorato, A Valdez… - Infection and …, 2024 - Am Soc Microbiol
Small molecules are components of fungal extracellular vesicles (EVs), but their biological
roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity …