Building on the successful structure of the first edition, the second edition of Reproductive
Technologies in Farm Animals has been totally updated and revised to provide an up to date …

Previous work has shown that activin A is expressed selectively within the theca rather than
the granulosa layer of preovulatory chicken follicles. In the present study, this finding was …

In general, oocytes arrested at metaphase of the second meiotic division (MII) are used as
recipient cytoplasts for nuclear transfer (NT) procedures. MII oocytes contain high levels of …

Cryopreservation of mature oocytes and embryos has provided numerous benefits in
reproductive medicine. Although successful cryopreservation of germinal-vesicle stage (GV) …

The yield efficiency of transgenic animal generation is relatively low. To improve its
efficiency has become a priority task for researchers. Melatonin (N-acetyl-5 …

Cloning by somatic cell nuclear transfer (SCNT) subverts sperm-mediated fertilization that
normally leads to physiological activation of the oocyte. Therefore, artificial activation is …

Zygotes have not been recognized as nuclear recipients since enucleated zygotes receiving
nuclei from beyond two-cell stage embryos are not able to form blastocysts. In the present …

The production of cloned animals is a difficult and complex procedure that requires two
basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes …

The present study demonstrated that demecolcine treatment for at least 30 min produces a
membrane protrusion in metaphase II–stage bovine oocytes. The maternal chromosome …

We generated a clone of bovine somatic cell nuclear transfer embryos using oocyte pools
from defined maternal sources to study nuclear-cytoplasmic interactions. Nucleocytoplasmic …