The rapid evolution of environmental (e) DNA methods has resulted in knowledge gaps in
smaller, yet critical details like proper use of negative controls to detect contamination …

Applications of environmental DNA (eDNA) analysis methods for biomonitoring have grown
exponentially over the last decade and provide a wealth of new information on the …

Misunderstandings regarding the term “false positive” present a significant hurdle to broad
adoption of eDNA monitoring methods. Here, we identify three challenges to clear …

Delineating conservation units (CUs, eg, evolutionarily significant units, ESUs, and
management units, MUs) is critical to the recovery of declining species because CUs inform …

Many species of amphibians in Northern Europe are threatened and the local distributions
are rarely described in detail. Application of modern molecular methods provides an …

To achieve reliable detection of environmental DNA (eDNA) from multiple species, a method
which combines the specificity of single-taxon assays with the broad taxonomic coverage of …

For nearly 15 years now, environmental DNA has demonstrated its effectiveness in
monitoring biodiversity. Methodological and technical improvements have significantly …

Invasive species and emerging infectious diseases are two of the greatest threats to
biodiversity. American Bullfrogs (Rana [Lithobates] catesbeiana), which have been …

Widespread amphibian declines were well documented at the end of the 20th century,
raising concerns about the need to identify individual and interactive contributors to this …

Environmental DNA (eDNA) monitoring of species distribution has become a critical tool in
ecology, conservation biology and fisheries for identifying the presence and distribution of …