Methodological approaches in vitrification: Enhancing viability of bovine oocytes and in vitro‐produced embryos

T Mogas, T García‐Martínez… - … in Domestic Animals, 2024 - Wiley Online Library
Cryopreservation of bovine oocytes and embryos is essential for long‐term preservation and
widespread distribution of genetic material, particularly in bovine in vitro embryo production …

Exopolysaccharide ID1 improves post-warming outcomes after vitrification of in vitro-produced bovine embryos

EA Ordóñez-León, I Martínez-Rodero… - International journal of …, 2022 - mdpi.com
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1,
produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine …

Blastocoel fluid aspiration improves vitrification outcomes and produces similar sexing results of in vitro-produced cattle embryos compared to microblade biopsy

I Martínez-Rodero, A Salas-Huetos, J Diaz-Muñoz… - Theriogenology, 2024 - Elsevier
The potential applications of in vitro-produced (IVP) cattle embryos are significantly
enhanced when combined with genotype selection and cryopreservation techniques. While …

Vitrification and in-straw warming do not affect pregnancy rates of biopsied bovine embryos

N González-Rodríguez, I Martínez-Rodero, J Scherzer… - Theriogenology, 2022 - Elsevier
In the cattle industry, in vivo or in vitro embryo production combined with genotyping and
cryopreservation technologies allows the selection and conservation of embryos carrying …

Use of Antifreeze Protein from Tenebrio molitor (TmAFP) in Vitrification of In Vitro–Produced Bovine Embryos: An Ultrastructural Study

RA Silva Júnior, R Desenzi, MMS Ramires… - Biopreservation and …, 2024 - liebertpub.com
The objective of this study was to evaluate the effects of different concentrations of antifreeze
protein (AFP) extracted from the larva of the beetle, Tenebrio molitor (Tm AFP), on …

Development of optimized vitrification procedures using closed carrier system to improve the survival and developmental competence of vitrified mouse oocytes

JK Park, JH Lee, EA Park, HJ Lim, SW Lyu, WS Lee… - Cells, 2021 - mdpi.com
The open carrier system (OC) is used for vitrification due to its high efficiency in preserving
female fertility, but concerns remain that it bears possible risks of cross-contamination …

[HTML][HTML] Impact of equilibration duration combined with temperature on the outcome of bovine oocyte vitrification

T García-Martínez, I Martínez-Rodero, J Roncero-Carol… - Theriogenology, 2022 - Elsevier
The cryopreservation of mammalian oocytes and embryos has become an integral part of
assisted reproduction in both humans and veterinary species. However, the methods used …

In silico-designed vitrification protocols: an approach to improve survival of in vitro produced-bovine embryos

I Martínez-Rodero, J Diaz-Muñoz, AZ Higgins… - …, 2024 - rep.bioscientifica.com
In brief In silico predictions validated in this study demonstrate the potential for designing
shorter equilibration protocols that improve post-warming re-expansion and hatching rates …

Fitness of calves born from in vitro-produced fresh and cryopreserved embryos

E Gómez, A Murillo, S Carrocera… - Frontiers in Veterinary …, 2022 - frontiersin.org
In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos,
differ in their physiology and survival from fresh embryos. In this study, we analyzed the …

Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos

I Martínez‐Rodero, A Salas‐Huetos… - … in Domestic Animals, 2022 - Wiley Online Library
The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular
exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to …