Lipid membrane mimetics in functional and structural studies of integral membrane proteins
Integral membrane proteins (IMPs) fulfill important physiological functions by providing cell–
environment, cell–cell and virus–host communication; nutrients intake; export of toxic …
environment, cell–cell and virus–host communication; nutrients intake; export of toxic …
[HTML][HTML] Structure and function of membrane proteins encapsulated in a polymer-bound lipid bilayer
NL Pollock, SC Lee, JH Patel, AA Gulamhussein… - … et Biophysica Acta (BBA …, 2018 - Elsevier
New technologies for the purification of stable membrane proteins have emerged in recent
years, in particular methods that allow the preparation of membrane proteins with their …
years, in particular methods that allow the preparation of membrane proteins with their …
[HTML][HTML] Crystallogenesis of membrane proteins mediated by polymer-bounded lipid nanodiscs
J Broecker, BT Eger, OP Ernst - Structure, 2017 - cell.com
For some membrane proteins, detergent-mediated solubilization compromises protein
stability and functionality, often impairing biophysical and structural analyses. Hence …
stability and functionality, often impairing biophysical and structural analyses. Hence …
[HTML][HTML] Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure
The field of membrane protein structural biology has been revolutionized over the last few
years with a number of high profile structures being solved using cryo-EM including Piezo …
years with a number of high profile structures being solved using cryo-EM including Piezo …
Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein
Biological characterisation of membrane proteins lags behind that of soluble proteins. This
reflects issues with the traditional use of detergents for extraction, as the surrounding lipids …
reflects issues with the traditional use of detergents for extraction, as the surrounding lipids …
Characterization of nanodisc-forming peptides for membrane protein studies
B Krishnarjuna, G Sharma, SC Im, R Auchus… - Journal of Colloid and …, 2024 - Elsevier
Lipid-bilayer nanodiscs provide a stable, native-like membrane environment for the
functional and structural studies of membrane proteins and other membrane-binding …
functional and structural studies of membrane proteins and other membrane-binding …
Enhancing the stability and homogeneity of non-ionic polymer nanodiscs by tuning electrostatic interactions
The nanodisc technology is increasingly used for structural studies on membrane proteins
and drug delivery. The development of synthetic polymer nanodiscs and the recent …
and drug delivery. The development of synthetic polymer nanodiscs and the recent …
Fast collisional lipid transfer among polymer-bounded nanodiscs
Some styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to
form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs …
form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs …
Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano …
RL Grime, J Goulding, R Uddin, LA Stoddart, SJ Hill… - Nanoscale, 2020 - pubs.rsc.org
The fundamental importance of membrane proteins in cellular processes has driven a
marked increase in the use of membrane mimetic approaches for studying and exploiting …
marked increase in the use of membrane mimetic approaches for studying and exploiting …
Influence of Poly(styrene-co-maleic acid) Copolymer Structure on the Properties and Self-Assembly of SMALP Nanodiscs
Polymer stabilized nanodiscs are self-assembled structures composed of a polymer belt that
wraps around a segment of lipid bilayer, and as such are capable of encapsulating …
wraps around a segment of lipid bilayer, and as such are capable of encapsulating …