Bromelain-loaded nanoparticles: A comprehensive review of the state of the art

JA Ataide, EF Gérios, PG Mazzola, EB Souto - Advances in Colloid and …, 2018 - Elsevier
Stem bromelain is a common available cysteine protease derived from pineapple (Ananas
comosus L.). Bromelain finds widespread applications in several areas, such as medicine …

Expanding the potential role of deep eutectic solvents toward facilitating the structural and thermal stability of α-chymotrypsin

N Yadav, K Bhakuni, M Bisht, I Bahadur… - ACS Sustainable …, 2020 - ACS Publications
Since the introduction of deep eutectic solvents (DESs), numerous reports have surfaced
describing its tunable properties and environmentally benign nature. Due to such favorable …

Lower kinetic limit to protein thermal stability: a proposal regarding protein stability in vivo and its relation with misfolding diseases

IM Plaza del Pino, B Ibarra‐Molero… - Proteins: Structure …, 2000 - Wiley Online Library
In vitro thermal denaturation experiments suggest that, because of the possibility of
irreversible alterations, thermodynamic stability (ie, a positive value for the unfolding Gibbs …

Bromelain partitioning in two-phase aqueous systems containing PEO–PPO–PEO block copolymers

APB Rabelo, EB Tambourgi, A Pessoa Jr - Journal of Chromatography B, 2004 - Elsevier
Bromelain is an enzymatic complex obtained from pineapple (Ananas comosus) fruits and
stem. Thermoseparation of bromelain by poly (ethylene oxide)(PEO)–poly (propylene …

Cisplatin bioconjugated enzymatic GNPs amplify the effect of cisplatin with acquiescence

S Iram, M Zahera, I Wahid, A Baker, M Raish, A Khan… - Scientific Reports, 2019 - nature.com
Enzymatic gold nanoparticles (B-GNPs) have been synthesized using a natural anticancer
agent bromelain (a cysteine protease) and these nanoparticles were used to bioconjugate …

Kinetic role of electrostatic interactions in the unfolding of hyperthermophilic and mesophilic rubredoxins

S Cavagnero, DA Debe, ZH Zhou, MWW Adams… - Biochemistry, 1998 - ACS Publications
The temperature dependence of the unfolding kinetics of rubredoxins from the
hyperthermophile Pyrococcus furiosus (RdPf) and the mesophile Clostridium pasteurianum …

Analytical techniques used to study the degradation of proteins and peptides: physical instability

JLE Reubsaet, JH Beijnen, A Bult… - … of pharmaceutical and …, 1998 - Elsevier
The physical instability of proteins and peptides as well as the various analytical techniques
used to study the various aspects of physical instability have been reviewed. Physical …

Effect of pH and temperature on the activity of enzymatic extracts from pineapple peel

MPC Silvestre, RL Carreira, MR Silva… - Food and Bioprocess …, 2012 - Springer
This study was carried out to characterize a crude extract from pineapple peel after
precipitation by three methods with the aim of obtaining an enzymatic extract from agro …

Characterization of a partially folded intermediate of stem bromelain at low pH

SK Haq, S Rasheedi, RH Khan - European Journal of …, 2002 - Wiley Online Library
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4. 22.32)
were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of …

Purification and characterization of bromelain from pineapple (Ananas comosus L.) peel waste

W Zhou, C Ye, L Geng, G Chen, X Wang… - Journal of Food …, 2021 - Wiley Online Library
Bromelain is widely used in food industry and pharmaceutical products due to its strong
antioxidant properties. Therefore, the extraction of bromelain from pineapple peel may …