Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
Evaluating and enhancing target specificity of gene-editing nucleases and deaminases
Programmable nucleases and deaminases, which include zinc-finger nucleases,
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
Potent and uniform fetal hemoglobin induction via base editing
Inducing fetal hemoglobin (HbF) in red blood cells can alleviate β-thalassemia and sickle
cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor …
cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor …
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Broad use of CRISPR–Cas12a (formerly Cpf1) nucleases has been hindered by the
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
Inference of CRISPR edits from Sanger trace data
Efficient and precise genome editing requires a fast, quantitative, and inexpensive assay to
assess genotype following editing. Here, we present ICE (Inference of CRISPR Edits), which …
assess genotype following editing. Here, we present ICE (Inference of CRISPR Edits), which …
Identifying cell receptors for the nanoparticle protein corona using genome screens
Nanotechnology provides platforms to deliver medical agents to specific cells. However, the
nanoparticle's surface becomes covered with serum proteins in the blood after …
nanoparticle's surface becomes covered with serum proteins in the blood after …
Gene correction for SCID-X1 in long-term hematopoietic stem cells
M Pavel-Dinu, V Wiebking, BT Dejene, W Srifa… - Nature …, 2019 - nature.com
Gene correction in human long-term hematopoietic stem cells (LT-HSCs) could be an
effective therapy for monogenic diseases of the blood and immune system. Here we …
effective therapy for monogenic diseases of the blood and immune system. Here we …
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions
How early events in effector T cell (T EFF) subsets tune memory T cell (T MEM) responses
remains incompletely understood. Here, we systematically investigated metabolic factors in …
remains incompletely understood. Here, we systematically investigated metabolic factors in …
Precise genome engineering in Drosophila using prime editing
JA Bosch, G Birchak… - Proceedings of the …, 2021 - National Acad Sciences
Precise genome editing is a valuable tool to study gene function in model organisms. Prime
editing, a precise editing system developed in mammalian cells, does not require double …
editing, a precise editing system developed in mammalian cells, does not require double …
[HTML][HTML] A genetically engineered primary human natural killer cell platform for cancer immunotherapy
EJ Pomeroy, JT Hunzeker, MG Kluesner, WS Lahr… - Molecular Therapy, 2020 - cell.com
Enhancing natural killer (NK) cell cytotoxicity by blocking inhibitory signaling could lead to
improved NK-based cancer immunotherapy. Thus, we have developed a highly efficient …
improved NK-based cancer immunotherapy. Thus, we have developed a highly efficient …