Transmission of arthropod-borne viruses (arboviruses) involves infection and replication in
both arthropod vectors and vertebrate hosts. Nearly all arboviruses are RNA viruses with …

Mosquito-borne diseases having the greatest impact on human health are typically
prevalent in the tropical belt of the world. However, these diseases are conquering …

How viruses evolve within hosts can dictate infection outcomes; however, reconstructing this
process is challenging. We evaluate our multiplexed amplicon approach, PrimalSeq, to …

Dengue virus causes approximately 96 million symptomatic infections annually, manifesting
as dengue fever or occasionally as severe dengue,. There are no antiviral agents available …

The emergence of mosquito-borne RNA viruses, such as West Nile virus (WNV), is facilitated
by genetically complex virus populations within hosts. Here, we determine whether WNV …

Due to their error-prone replication, RNA viruses typically exist as a diverse population of
closely related genomes, which is considered critical for their fitness and adaptive potential …

Recent field trials have demonstrated that dengue incidence can be substantially reduced
by introgressing strains of the endosymbiotic bacterium Wolbachia into Aedes aegypti …

Summary Arthropod-borne viruses (arboviruses), such as Zika virus, chikungunya virus, and
West Nile virus (WNV), pose continuous threats to emerge and cause large epidemics …

The incidence and geographic range of dengue have increased dramatically in recent
decades. Climate change, rapid urbanization and increased global travel have facilitated the …

Highlights•RNA virus replication gives rise to a cloud of variants containing mutations,
insertions and deletions, some of which contribute to evolution.•Virus population diversity …